Zobrazeno 1 - 10
of 260
pro vyhledávání: '"TA cloning"'
Publikováno v:
BioTechniques, Vol 68, Iss 5, Pp 251-256 (2020)
The establishment of transgenic plants has greatly promoted the progress of plant research. However, traditional selection methods using antibiotics or herbicides may miss any positive transformants with growth defects. Additionally, screening with a
Externí odkaz:
https://doaj.org/article/3162b6e0c64e48c0bd6e746df603a875
Publikováno v:
Frontiers in Marine Science, Vol 8 (2022)
The small yellow croaker, Larimichthys polyactis, is a keystone species in the Yellow Sea and the East China Sea, with significant impacts on the regional ecosystem, but has experienced decades of population decline as a result of environmental chang
Externí odkaz:
https://doaj.org/article/d1f251f37aed41f7a968e990d36a11c3
Publikováno v:
Journal of Integrative Neuroscience, Vol 18, Iss 4, Pp 341-350 (2019)
The γ-Aminobutyric acid type A receptors (GABAARs) are heteropentameric chloride channels responsible for primary inhibition in the mammalian brain. Studies have shown the expression of recombinant GABAAR subunits tagged with the green fluorescent p
Externí odkaz:
https://doaj.org/article/4a57e44d59d34e399d6c6f81b1606590
Publikováno v:
Archives of Razi Institute, Vol 73, Iss 2, Pp 107-111 (2018)
Iota toxin is produced by Clostridium perfringens type E. This toxin causes antibiotic-associated enterotoxemia in lambs and calves. Iota toxin is a binary toxin that has two components including Ia (the enzyme component) and Ib (the binding componen
Externí odkaz:
https://doaj.org/article/de35557be94f4278acd170b112fc9a40
Autor:
Seong Min Kim, Myung Jun Kim, Myung Hee Yi, Ju Yeong Kim, Joon Sup Yeom, Tai Soon Yong, Hyun Dong Yoo
Publikováno v:
Annals of Laboratory Medicine. 42:196-202
Background Identifying the causal pathogen of encephalitis remains a clinical challenge. A 50-year-old man without a history of neurological disease was referred to our department for the evaluation of an intracranial lesion observed on brain magneti
Publikováno v:
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 987-991 (2015)
Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. Methods: In this research, 5′ upstream and 3′ downstream regions of S. typhimurium sipC gene and kanamycin gene wer
Externí odkaz:
https://doaj.org/article/e54f31aad37645ddae310c0efeba7435
Publikováno v:
Journal of Global Antimicrobial Resistance, Vol 26, Iss, Pp 66-68 (2021)
Objectives The aim of this study was to characterise a tigecycline-resistant blaNDM-9-bearing Klebsiella aerogenes strain (HNHF1) of chicken origin. Methods Strain HNHF1 was characterised by phenotypic antimicrobial susceptibility testing, PCR, conju
Autor:
Haruhiko Sugimura, Ken Sugimoto, Shinya Tani, Terumi Taniguchi, Moriya Iwaizumi, Masato Maekawa, Takahisa Furuta, Hiroaki Miyajima, Satoshi Osawa, Yasushi Hamaya, Tomohiro Sugiyama, Satoshi Baba, Mihoko Yamade, Satoshi Suzuki, Tsutomu Ohta
Publikováno v:
Journal of Clinical Pathology. 74:386-390
AimsAlthough frameshift variants in the microsatellite area of shugoshin 1 (SGO1) have been reported in the context of microsatellite instability-high (MSI-H)/deficient mismatch repair gastrointestinal cancer, most have been evaluated only in early s
Publikováno v:
Nucleic Acids Research
The dynamic topological structure of telomeric DNA is closely related to its biological function; however, no such structural information on full-length telomeric DNA has been reported due to difficulties synthesizing long double-stranded telomeric D
Publikováno v:
International Journal of Chemical Studies. 8:649-655
The present experiment was aimed to detect osteopontin gene transcript from bull spermatozoa. Fresh semen samples from 12 Jersey cross breed bulls were collected using artificial vagina. The volume and concentration of the individual samples were rec