Zobrazeno 1 - 10
of 67
pro vyhledávání: '"T.R Doel"'
Autor:
J Baron, T.R Doel, A Childerstone, R. M. E. Parkhouse, Mike Flint, T. Collen, Martin D. Ryan, A Corteyn, M. Garcia-Valcarcel
Publikováno v:
Virus Research. 56:125-133
In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3Dpol by PBMC and CD4+ T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d11d3243db96e97494f091d266d6a08
https://doi.org/10.1016/s0168-1702(98)00035-5
https://doi.org/10.1016/s0168-1702(98)00035-5
Publikováno v:
Vaccine. 12:592-600
Emergency foot-and-mouth disease (FMD) vaccines prepared from antigens held in the International Vaccine Bank at Pirbright were administered to cattle and pigs and the levels of protection were assessed following challenge by contact with infected pi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94e850e538dbf901041eef96b575bdd1
https://doi.org/10.1016/0264-410x(94)90262-3
https://doi.org/10.1016/0264-410x(94)90262-3
Publikováno v:
Vaccine. 11:415-421
The polymerase chain reaction method (PCR) has been applied to the diagnosis of foot-and-mouth disease viral RNA in tissues and, particularly, oesophageal-pharyngeal fluid (probang) samples from cattle. Using primer sets which corresponded to conserv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13899c81ba1a008355196e2feea50397
https://doi.org/10.1016/0264-410x(93)90281-2
https://doi.org/10.1016/0264-410x(93)90281-2
Publikováno v:
Vaccine. 8(5)
An international bank for foot-and-mouth disease (FMD) vaccine has been established at the Pirbright Laboratory of the AFRC Institute for Animal Health. The bank is based on concentrated virus preparations stored in the gaseous phase of liquid nitrog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1eee6ebc53855707e697217cf04060e
https://pubmed.ncbi.nlm.nih.gov/2174597
https://pubmed.ncbi.nlm.nih.gov/2174597
Publikováno v:
Journal of Biological Standardization. 10:69-81
The integrity of VP1 in conventional and concentrated preparations of foot-and-mouth disease virus was examined. In general, degradation of VP1 was only observed in concentrated preparations stored at 4°C. When added to concentrates, serum or Trasyl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddc67479027559c7ff2975598f377b0e
https://doi.org/10.1016/s0092-1157(82)80028-0
https://doi.org/10.1016/s0092-1157(82)80028-0
Autor:
T.R. Doel, G.N. Mowat
Publikováno v:
Journal of biological standardization. 13(4)
Workers in 11 laboratories in Europe and one in North America participated in a collaborative study to assess the variability of a sucrose gradient procedure used for the quantification of foot and mouth disease virus (FMDV). To this end, a range of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a865a95b83fa952ce251475fc4606baa
https://pubmed.ncbi.nlm.nih.gov/2997228
https://pubmed.ncbi.nlm.nih.gov/2997228
Autor:
T.R. Doel, Deborah J. David
Publikováno v:
Journal of biological standardization. 12(3)
The stability of 146S particles in concentrates of foot-and-mouth disease virus stored at 4 degrees C was similar to that of 146S particles in a conventional virus preparation. Proteolytic degradation of VPl was not observed in the stored conventiona
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5138d841298cc65c1eb6c03b10116cac
https://pubmed.ncbi.nlm.nih.gov/6090464
https://pubmed.ncbi.nlm.nih.gov/6090464
Publikováno v:
Journal of biological standardization. 12(1)
The use of a density gradient procedure for the quantification of intact, inactivated poliovirus particles in vaccine preparations is described. The procedure is both sensitive and highly reproducible and the results correlate with those of potency t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::223f1e7052a7b2212d19d7503cb3ff3c
https://pubmed.ncbi.nlm.nih.gov/6699027
https://pubmed.ncbi.nlm.nih.gov/6699027
Publikováno v:
Journal of virological methods. 7(4)
A method for the isolation of foot-and-mouth disease virus (FMDV) capsid proteins was developed. The FMDV capsid proteins VP1, VP2, VP3 and VP0 were isolated from sucrose gradient purified virus by chromatofocusing in a pH 7.4-4.0 gradient on Polybuf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7907edd1dc63a9104db72b931e2cf101
https://pubmed.ncbi.nlm.nih.gov/6317707
https://pubmed.ncbi.nlm.nih.gov/6317707
Autor:
T.R. Doel, R.F. Staple
Publikováno v:
Journal of biological standardization. 10(2)
Saponin used at a concentration of 1·7 mg ml −1 caused O BFS 1860, A Cruzeiro and SAT 3 Bec 1/65 to aggregate, whereas viruses of the C serotype, notably C Noville, were not affected. Studies with radioactive viruses indicated that crude saponin d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba6c9d545548da567d5d77e9341a96d1
https://pubmed.ncbi.nlm.nih.gov/6284760
https://pubmed.ncbi.nlm.nih.gov/6284760