Zobrazeno 1 - 10
of 14
pro vyhledávání: '"T. V. Chepurnykh"'
Autor:
Ilia V. Yampolsky, A. S. Gerasimov, Andrey Yu. Gorokhovatsky, S. O. Rogozhkin, T. V. Chepurnykh, N M Myshkina, Ekaterina S. Shakhova, A. V. Balakireva
Publikováno v:
Russian Journal of Bioorganic Chemistry. 47:1066-1076
Bioluminescence is a phenomenon of light emission resulting from oxidation of a substrate, luciferin, catalyzed by the enzyme luciferase. The fungus Neonothopanus nambi is the first eukaryotic organism with a fully deciphered bioluminescent system: t
Autor:
Valentin N. Petushkov, Natalja S. Rodionova, Ilia V. Yampolsky, K. V. Purtov, T. V. Chepurnykh, A A Kotlobay, N M Myshkina, K A Beregovaya
Publikováno v:
Doklady Biochemistry and Biophysics. 496:14-17
The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi
Autor:
S. A. Goncharuk, M. N. Baranova, A. S. Shcheglov, A. Yu. Gorokhovatsky, Ilia V. Yampolsky, T. V. Chepurnykh, K. V. Purtov, Natalja S. Rodionova, Valentin N. Petushkov, Yu. A. Mokrushina
Publikováno v:
Doklady. Biochemistry and Biophysics
Abstract A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autolumi
Autor:
T. V. Chepurnykh, A. S. Shcheglov, K. V. Purtov, RI Zagitova, RH Ziganshin, Natalja S. Rodionova, VB Kozhemyako, Valentin N. Petushkov, Aleksandra S. Tsarkova
Publikováno v:
Bulletin of Russian State Medical University.
The marine polychaete Chaetopterus variopedatus (Renier) (family Chaetopteridae) is a cosmopolitan species complex, consisting of distinct populations/ subspecies. The worms release glowing (460 nm) clouds of mucus when disturbed, and their parapodia
Autor:
K A, Beregovaya, N M, Myshkina, T V, Chepurnykh, A A, Kotlobay, K V, Purtov, V N, Petushkov, N S, Rodionova, I V, Yampolsky
Publikováno v:
Doklady. Biochemistry and biophysicsREFERENCES. 496(1)
The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi
Autor:
Natalja S. Rodionova, A A Kotlobay, K. V. Purtov, Z. M. Osipova, A. Yu. Gorokhovatsky, Valentin N. Petushkov, Ilia V. Yampolsky, J. I. Gitelson, Aleksandra S. Tsarkova, T. V. Chepurnykh
Publikováno v:
Doklady Biochemistry and Biophysics. 480:177-180
This is the first study to obtain a high-purity luciferase from the fungus Neonothopanus nambi biomass that is suitable for subsequent sequencing.
Autor:
Natalja S. Rodionova, K. V. Purtov, A A Kotlobay, Valentin N. Petushkov, J. I. Gitelson, Z. M. Osipova, A. Yu. Gorokhovatsky, Ilia V. Yampolsky, T. V. Chepurnykh, Aleksandra S. Tsarkova
Publikováno v:
Doklady. Biochemistry and biophysics. 477(1)
The structure of fungal oxyluciferin was determined, the enzymatic bioluminescence reaction under substrate saturation conditions with discrete monitoring of formed products was conducted, and the structures of the end products of the reaction were e
Autor:
Konstantin A. Lukyanov, Vladimir Z. Pletnev, Alexander Wlodawer, Sergei Pletnev, Ekaterina A. Souslova, Nadya V. Pletneva, T. V. Chepurnykh, Dmitry M. Chudakov, Zbigniew Dauter, Ilia V. Yampolsky, Arkady F. Fradkov
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 69:1850-1860
A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cni
Autor:
T. V. Chepurnykh, Dmitriy M. Chudakov, Sergey Lukyanov, Vsevolod V. Belousov, Konstantin A. Lukyanov
Publikováno v:
Traffic. 7:1304-1310
Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here
Autor:
T. V. Chepurnykh, Sergey Lukyanov, Konstantin A. Lukyanov, Maria Shkrob, Yurii G. Yanushevich, Olga V. Britanova, Dmitry B. Staroverov, Dmitriy M. Chudakov, Ekaterina M. Merzlyak, Maria E Bulina
Publikováno v:
Nature Biotechnology. 24:95-99
Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodyn