Výsledky vyhledávání - "T. O. Guseinov"

A Simultaneous Use of Cy5-Modified Derivatives of Deoxyuridine and Deoxycytidine in PCR

Autor: V. E. Kuznetsova, Alexander V. Chudinov, A. S. Pavlov, T. O. Guseinov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Shershov

Publikováno v: Russian Journal of Bioorganic Chemistry. 46:557-562

Particular features of the simultaneous incorporation into the growing DNA chain of modified Cy5-deoxyuridines (Cy5-dU) and deoxycytidines (Cy5-dC) and Taq DNA polymerase in the process of PCR amplification were studied. The studies were carried out

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a3d459d7fccb8a123ee3a804be6c5942
https://doi.org/10.1134/s1068162020040111

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Amplification Efficiency and Substrate Properties of Fluorescently Labeled Deoxyuridine Triphosphates in PCR in the Presence of DNA Polymerases without 3'-5' Exonuclease Activity

Autor: V. E. Kuznetsova, Alexander S. Zasedatelev, Sergey A. Lapa, M. A. Spitsyn, O. S. Volkova, Alexander V. Chudinov, V. E. Shershov, T. O. Guseinov

Publikováno v: Russian Journal of Bioorganic Chemistry. 45:263-272

The kinetics of PCR amplification has been studied using fluorescently labeled zwitterionic deoxyuridine triphosphates that contain different fluorophores linkers of different lengths between the fluorophore and nitrogenous base. The concentration de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::84312f0671eb6d192a98b1409830fd45
https://doi.org/10.1134/s1068162019040046

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Ферментативное получение модифицированных ДНК: изучение кинетики ПЦР в режиме реального времени

Autor: S. P. Radko, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, A. V. Lisitsa, V. E. Shershov, A S Pavlov, M. A. Spitsyn, T. O. Guseinov, Alexander V. Chudinov

Publikováno v: Молекулярная биология. 53:513-523

The effects of modified deoxyuridine triphosphates (mod-dUTPs) with different substituents at the C5 position of the pyrimidine cycle on the kinetics of PCR with Taq and Vent (exo-) DNA polymerases are studied. Substituents in mod-dUTP include carbox

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::773cd0c3a230447917f5be5445dd7f2a
https://doi.org/10.1134/s0026898419030091

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Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation

Autor: A. V. Lisitsa, Edward N. Timofeev, Sergey A. Lapa, Alexander V. Chudinov, K. S. Romashova, M. A. Spitsyn, V. E. Kuznetsova, V. E. Shershov, O. A. Zasedateleva, T. O. Guseinov, Sergey P. Radko

Publikováno v: Molecular Biology. 52:854-864

A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with the simultaneous incorporation of modified nucleotides and subsequent separation of DNA stran

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::750f91d808dc0aa933976b0e87e5a42c
https://doi.org/10.1134/s0026893318060110

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Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy

Autor: Edward N. Timofeev, T. O. Guseinov, Alexander V. Chudinov, V. E. Barsky, M. A. Spitsyn, Yu. P. Lysov, O. A. Zasedateleva, D. D. Mamaev, V. E. Shershov, R. A. Miftakhov, V. E. Kuznetsova, Alexander S. Zasedatelev, Sergey A. Lapa

Publikováno v: Biophysics. 63:512-518

A method to estimate the concentration of chemically accessible carboxylic groups on the surface of a polyethylene terephthalate (PET) film was developed using the fluorochrome cycanine Cy5 and digital fluorescence microscopy. A method was developed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8e25609beaec9c39b08b74984630f356
https://doi.org/10.1134/s0006350918040127

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Synthetic route to novel zwitterionic pentamethine indocyanine fluorophores with various substitutions

Autor: Alexander S. Zasedatelev, V. E. Shershov, Sergey A. Lapa, T. O. Guseinov, V. E. Kuznetsova, Мarina А. Emelyanova, M. A. Spitsyn, Alexander V. Chudinov, Tatyana V. Nasedkina

Publikováno v: Dyes and Pigments. 147:199-210

Two series of novel zwitterionic water-soluble pentamethine cyanine dyes with geometrically balanced structures were synthesized and characterized. One series of cyanine dyes is the «zwitterionic» fluorophores, which contain positively charged trim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4a9a61732fd5b5a9ffba9a7b3faa1916
https://doi.org/10.1016/j.dyepig.2017.07.052

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The Effect of the Structure of Fluorescently Labeled Nucleotide Derivatives on the Efficiency of Their Incorporation in DNA in the Polymerase Chain Reaction

Autor: Alexander V. Chudinov, A. Yu. Ikonnikova, V. E. Shershov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, T. S. Lisitsa, D. O. Fesenko, T. O. Guseinov, M. A. Spitsyn, T. V. Nasedkina

Publikováno v: Biophysics. 62:900-904

The efficiency of fluorescence DNA labeling was estimated for four fluorescent 2′-deoxyuridine 5′-triphosphate derivatives differing in the orientation of the main dye axis, which passes through the polymethine chain, relative to the linker conne

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c4b03b14b48a55e70e8a56dc4b074bed
https://doi.org/10.1134/s0006350917060082

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