Zobrazeno 1 - 10
of 212
pro vyhledávání: '"T. Muiño-Blanco"'
Autor:
I, Barranco, A, Casao, C, Perez-Patiño, I, Parrilla, T, Muiño-Blanco, E A, Martinez, J A, Cebrian-Perez, J, Roca
Publikováno v:
Journal of animal science. 95(4)
Melatonin (MLT) is present in seminal plasma (SP) of mammalian species, including pigs, and it is credited with antioxidant properties. This study aims to identify the sources of variation and the role of boar SP MLT on sperm quality and functionalit
Publikováno v:
Theriogenology. 46:181-190
Lectin binding is an accepted procedure for studying surface properties of gamete membranes. The procedure has not been widely used in invertebrates. Glycoproteins present on cell membranes in spermatozoa of Apis mellifera L. drones, may play a role
Publikováno v:
Bioscience Reports. 16:35-40
Centrifugal countercurrent distribution (CCCD) in aqueous two-phase systems has been proven to be a useful method to study subtle surface properties of spermatozoa. The present work shows that a short-term inhibition of the energy metabolism of sperm
Publikováno v:
Reproduction in domestic animals = Zuchthygiene. 45(6)
The objectives of this study were two. First, to compare three base media with different sugar composition as an initial step to achieve a good chemically-defined extender for ram sperm refrigeration. The second one, to determine which sperm quality
Publikováno v:
International journal of andrology. 33(1)
We have previously shown that a cocktail-containing phosphodiesterase inhibitors (theophylline and caffeine), a phosphatase inhibitor (okadaic acid) and dibutyryl-cAMP promoted specific protein tyrosine phosphorylation in ram spermatozoa during incub
Autor:
A, Casao, S, Vega, I, Palacín, R, Pérez-Pe, A, Laviña, F J, Quintín, E, Sevilla, J A, Abecia, J A, Cebrián-Pérez, F, Forcada, T, Muiño-Blanco
Publikováno v:
Reproduction in domestic animals = Zuchthygiene. 45(3)
The effect of melatonin implants administered during non-breeding season in Rasa Aragonesa rams on sperm motility parameters and other reproductive traits was assessed. In a first experiment, two Rasa Aragonesa rams were implanted (with melatonin gro
Autor:
José Antonio Enríquez, M. T. Muiño Blanco, M.J. López-Pérez, J. Hernandez-Yago, José Sánchez-Prieto
Publikováno v:
Journal of Neurochemistry. 55:1841-1849
Synaptosomes from rat forebrain can easily be isolated by combining centrifugation with partition in an aqueous two-phase system composed of dextran T500 and polyethylene glycol 4000 in which synaptosomes have an extreme affinity for the upper phase.
Publikováno v:
Biology of reproduction. 63(5)
Ejaculated ram spermatozoa, freed from seminal plasma by a dextran/swim-up procedure and exposed to cold shock, were incubated with ram seminal plasma proteins and analyzed by fluorescence markers and scanning electron microscopy. Seminal plasma prot
Publikováno v:
Journal of andrology. 21(4)
The acrosome reaction is an important marker for sperm function. Because different laboratory techniques may be used to detect this exocytotic process, the objective of this study was to investigate the use of fluoresceinated lectins to assess the ac
Publikováno v:
Journal of chromatography. B, Biomedical applications. 680(1-2)
Ram sperm was frozen in the presence of the most commonly used cryoprotectants. After thawing, the overall cell surface changes provoked by freezing were assessed by centrifugal counter-current distribution (CCCD). In addition, cell membrane integrit