Zobrazeno 1 - 9
of 9
pro vyhledávání: '"T. M. Timmons"'
Publikováno v:
Reproduction. 96:815-825
This study was designed to explore the composition of the equine zona pellucida (EZP) by one- and two-dimensional polyacrylamide gel electrophoresis (1D- and 2D-PAGE), silver staining and immunoblotting techniques. Antral follicles palpable on frozen
Publikováno v:
Biology of reproduction. 65(3)
A lactosaminoglycan-associated antigen is associated with a carbohydrate moiety of all three zona pellucida (ZP) glycoproteins of pig and rabbit but is absent in the mouse and rat. A monoclonal antibody (PS1) recognizing this determinant was obtained
Publikováno v:
The Journal of biological chemistry. 268(17)
A cDNA (rc75) encoding a 75-kDa rabbit zona pellucida (ZP) glycoprotein (R75) has been cloned and sequenced. The predicted amino acid sequence consists of 676 amino acids including seven potential N-glycosylation sites. The cDNA hybridizes to a 2.4-k
Autor:
E, Schwoebel, S, Prasad, T M, Timmons, R, Cook, H, Kimura, E M, Niu, P, Cheung, S, Skinner, S E, Avery, B, Wilkins
Publikováno v:
The Journal of biological chemistry. 266(11)
A full-length cDNA (rc55) encoding the major rabbit zona pellucida (ZP) glycoprotein (55 kDa) has been cloned and sequenced. A lambda gt11 expression library was constructed using poly(A)+ mRNA isolated from sexually immature rabbit ovaries which con
Publikováno v:
Biology of reproduction. 43(6)
A unique ovarian follicle cell culture system has been established to analyze the effects of extracellular matrix (ECM) on early granulosa cell differentiation. Primary and early secondary follicles isolated from ovaries of sexually immature rabbits
Autor:
T M, Timmons, B S, Dunbar
Publikováno v:
Methods in enzymology. 182
Publikováno v:
Journal of Biological Chemistry. 261:15385-15389
Rabbit skeletal muscle glycogen debranching enzyme is inactivated in a kinetically biphasic manner by GSSG at pH 8.0. The rapid phase results in the loss of 30% activity, while the slower phase leads to total enzyme inactivation. Both the glucosidase
Publikováno v:
The Journal of biological chemistry. 261(33)
Rabbit skeletal muscle glycogen debranching enzyme is inactivated in a kinetically biphasic manner by GSSG at pH 8.0. The rapid phase results in the loss of 30% activity, while the slower phase leads to total enzyme inactivation. Both the glucosidase
Publikováno v:
Biology of reproduction. 36(5)
Specific monoclonal and polyclonal antibodies to solubilized porcine and rabbit zonae pellucidae (ZP) and to purified ZP glycoprotein components have been used to define distinct ZP antigens. These studies demonstrate that the individual ZP glycoprot