Zobrazeno 1 - 3
of 3
pro vyhledávání: '"T. L. Eraizer"'
Autor:
N. I. Kuprina, T. L. Eraizer, G. I. Abelev, L. Ya. Shipova, A. B. Lunkina, V. S. Poltoranina, A. K. Yazova, N. L. Lazarevich
Publikováno v:
Bulletin of Experimental Biology and Medicine. 122:1115-1119
Rat hepatoma cell line McA Rh 7777 produces α-fetoprotein (AFP)in vitro. This line is subcloned into ATP-producing and nonproducing subclones. The AFP-negative clone is used to generate hybridomas producing monoclonal antibodies recognizing AFT− a
Publikováno v:
Bulletin of Experimental Biology and Medicine. 79:598-600
A simple and reproducible method of monolayer cultivation of hepatocytes from 7–12-week human fetuses is suggested. Such cultures are capable of limited growth, they can be maintained without subculaute for 4–6 weeks, and they preserve certain sp
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 83(4)
Alpha-fetoprotein (AFP) produced by individual hepatocytes and hepatocyte microcolonies was detected with microelectrophoresis-precipitation in polyacrylamide gel. Hepatic cells of 6--13-week-old human embryos were cultivated in vitro for 2 to 5 days