Zobrazeno 1 - 10
of 14
pro vyhledávání: '"T. K. Christopoulos"'
Publikováno v:
The Analyst. 123:1315-1319
We report microtiter well-based sandwich-type DNA hybridization assays using enzyme amplified time-resolved fluorometry of Tb3+ chelates. The target DNA was hybridized with two adjacent and non-overlapping oligonucleotide probes, one oligonucleotide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68a28a169d5b2e76353e63cc914b900e
https://doi.org/10.1039/a707528f
https://doi.org/10.1039/a707528f
Publikováno v:
The Analyst. 123:1309-1314
An immunoassay is reported which uses, as a label, an expressible DNA fragment encoding the alpha-peptide of beta-galactosidase. This inactive peptide consists of 97 amino acid residues containing an amino-terminal portion of the enzyme. Antigen (an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec43257424f0b882b8892143ae7f26e2
https://doi.org/10.1039/a706408j
https://doi.org/10.1039/a706408j
Autor:
N H, Chiu, T K, Christopoulos
Publikováno v:
Clinical chemistry. 45(11)
We report the first two-site, "sandwich type" expression immunoassay using as a label an expressible DNA fragment encoding firefly luciferase.The DNA label consisted of a T7 RNA polymerase promoter, a firefly luciferase-coding sequence, and a poly(dA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::41b362b2d69c0f04b3c198b065bdee70
https://pubmed.ncbi.nlm.nih.gov/10545065
https://pubmed.ncbi.nlm.nih.gov/10545065
Publikováno v:
Clinical chemistry. 44(6 Pt 1)
We report a quantitative analytical methodology for prostate-specific antigen (PSA) mRNA, which is based on the coamplification of the target with a recombinant RNA internal standard (IS) using reverse transcriptase-polymerase chain reaction. PSA mRN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e2e67058be8f01cbfc05e75a45bae60f
https://pubmed.ncbi.nlm.nih.gov/9625039
https://pubmed.ncbi.nlm.nih.gov/9625039
Autor:
S, Bortolin, T K, Christopoulos
Publikováno v:
Clinical chemistry. 42(12)
A microtiter well-based quantitative reverse transcriptase-PCR assay for determination of BCR-ABL mRNA, which relies on coamplification of the target with an RNA internal standard (IS), was developed. The hapten digoxigenin (Dig) is incorporated duri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0788f291d63dbd0680c4bb90867fe437
https://pubmed.ncbi.nlm.nih.gov/8969627
https://pubmed.ncbi.nlm.nih.gov/8969627
Publikováno v:
Clinical chemistry. 41(12 Pt 1)
We have developed a time-resolved fluorometric hybridization assay for detecting prostate-specific antigen (PSA) mRNA amplified by reverse transcription polymerase chain reaction. During PCR, digoxigenin-11-dUTP is incorporated into the amplified pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2fea29aaa6929779a050f8ce48244f45
https://pubmed.ncbi.nlm.nih.gov/7497609
https://pubmed.ncbi.nlm.nih.gov/7497609
Autor:
S, Bortolin, T K, Christopoulos
Publikováno v:
Clinical chemistry. 41(5)
Two hybridization assays have been developed to detect BCR-ABL mRNA transcripts arising from the Philadelphia translocation. Both assays use time-resolved immunofluorometric detection of polymerase chain reaction-amplified BCR-ABL mRNA sequences hybr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6a5c6439034a189d26f9760919502fc2
https://pubmed.ncbi.nlm.nih.gov/7729047
https://pubmed.ncbi.nlm.nih.gov/7729047
Publikováno v:
Clinical chemistry. 38(4)
We describe an ultrasensitive, enzymatically amplified time-resolved fluorescence immunoassay of thyrotropin (thyroid-stimulating hormone) in serum with use of a terbium chelate as the detectable moiety. In this assay, thyrotropin is first simultaneo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::51d46df611fb070d3461550d0b1f7212
https://pubmed.ncbi.nlm.nih.gov/1568319
https://pubmed.ncbi.nlm.nih.gov/1568319
Publikováno v:
Clinical chemistry. 38(3)
We describe a new multianalyte immunoassay principle and apply it to the simultaneous immunoassay of lutropin, follitropin, choriogonadotropin, and prolactin in serum. The method is based on the coating of distinct areas of polystyrene with analyte-s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f122ea1ff683ed5c7d59e66ae4b38649
https://pubmed.ncbi.nlm.nih.gov/1547545
https://pubmed.ncbi.nlm.nih.gov/1547545
Autor:
E P, Diamandis, T K, Christopoulos
Publikováno v:
BioTechniques. 10(5)
We use high-performance liquid chromatography with fraction collection to separate an antigen of interest. The antigen is then immobilized on polystyrene microtiter wells and detected with a specific antibody, followed by a second biotinylated antibo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7f43b5f0cee49dcbcd19048c61c53ee8
https://pubmed.ncbi.nlm.nih.gov/1716927
https://pubmed.ncbi.nlm.nih.gov/1716927