Single-cell ATAC and RNA sequencing reveal pre-existing and persistent cells associated with prostate cancer relapse

Autor: S. Taavitsainen, N. Engedal, S. Cao, F. Handle, A. Erickson, S. Prekovic, D. Wetterskog, T. Tolonen, E. M. Vuorinen, A. Kiviaho, R. Nätkin, T. Häkkinen, W. Devlies, S. Henttinen, R. Kaarijärvi, M. Lahnalampi, H. Kaljunen, K. Nowakowska, H. Syvälä, M. Bläuer, P. Cremaschi, F. Claessens, T. Visakorpi, T. L. J. Tammela, T. Murtola, K. J. Granberg, A. D. Lamb, K. Ketola, I. G. Mills, G. Attard, W. Wang, M. Nykter, A. Urbanucci

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)

Identifying the molecular mechanisms of response to systemic therapy in prostate cancer remains crucial. Here, the authors apply single cell-ATAC and RNAseq to models of early treatment response and resistance to enzalutamide and identify chromatin a

Externí odkaz: https://doaj.org/article/cc3e718f606845d686441f42a30442e4

Hairy Root Cultures—A Versatile Tool With Multiple Applications

Autor: Noemi Gutierrez-Valdes, Suvi T. Häkkinen, Camille Lemasson, Marina Guillet, Kirsi-Marja Oksman-Caldentey, Anneli Ritala, Florian Cardon

Publikováno v: Frontiers in Plant Science, Vol 11 (2020)

Hairy roots derived from the infection of a plant by Rhizobium rhizogenes (previously referred to as Agrobacterium rhizogenes) bacteria, can be obtained from a wide variety of plants and allow the production of highly diverse molecules. Hairy roots a

Externí odkaz: https://doaj.org/article/0a1d6325993d47da9a9df3fda54af15c

Inactivation of the germacrene A synthase genes by CRISPR/Cas9 eliminates the biosynthesis of sesquiterpene lactones in Cichorium intybus L

Autor: Ingrid M. van der Meer, Jules Beekwilder, Suvi T. Häkkinen, Johanna C. Hakkert, Dirk Bosch, Katarina Cankar, Robert Sevenier, Michiel Theodoor Jan De Both, Paul Bundock

Publikováno v: Plant Biotechnology Journal
Cankar, K, Bundock, P, Sevenier, R, Häkkinen, S T, Hakkert, J C, Beekwilder, J, van der Meer, I M, de Both, M & Bosch, D 2021, ' Inactivation of the germacrene A synthase genes by CRISPR/Cas9 eliminates the biosynthesis of sesquiterpene lactones in Cichorium intybus L. ', Plant Biotechnology Journal, vol. 19, no. 12, pp. 2442-2453 . https://doi.org/10.1111/pbi.13670
Plant Biotechnology Journal, 19(12), 2442-2453
Plant Biotechnology Journal 19 (2021) 12

Chicory (Cichorium intybus var. sativum) is an industrial crop species cultivated for the production of a fructose polymer inulin, which is used as a low‐calorie sweetener and prebiotic. Besides, inulin chicory taproots also accumulate sesquiterpen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c377b14acd732a24f3a04f2ace32680
https://cris.vtt.fi/en/publications/510f9619-8b84-4a7c-9466-c1f9741a1fe9

Plant cell cultures as food—aspects of sustainability and safety

Autor: Suvi T. Häkkinen, Raija-Liisa Heiniö, Heli Nygren, Natalia Maiorova, Anneli Ritala, Riitta Puupponen-Pimiä, Liisa Nohynek, Heiko Rischer

Publikováno v: Plant Cell Reports
Häkkinen, S T, Nygren, H, Nohynek, L, Puupponen-Pimiä, R, Heiniö, R L, Maiorova, N, Rischer, H & Ritala, A 2020, ' Plant cell cultures as food—aspects of sustainability and safety ', Plant Cell Reports, vol. 39, no. 12, pp. 1655–1668 . https://doi.org/10.1007/s00299-020-02592-2

Key message Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Abstract Biotechnologically produced cellul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aacdc1ce6e06d48896113cc61641cff1
http://europepmc.org/articles/PMC7644541

Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process

Autor: Noemi Gutierrez‐Valdes, Suvi T. Häkkinen, Camille Lemasson, Jonas de Groot, Jean‐Pierre Ele‐Ekouna, Marina Guillet, Florian Cardon, Anneli Ritala

Publikováno v: Biotechnology and Bioengineering
Gutierrez-Valdes, N, Häkkinen, S T, Lemasson, C, De Groot, J, Ele-Ekouna, JP, Guillet, M, Cardon, F & Ritala, A 2022, ' Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process ', Biotechnology and Bioengineering, vol. 119, no. 10, pp. 2831-2841 . https://doi.org/10.1002/bit.28178
Biotechnology and bioengineering
Biotechnology and bioengineering, 2022, ⟨10.1002/bit.28178⟩

International audience; Hairy root systems have proven to be a viable alternative for recombinant protein production. For recalcitrant proteins, maximizing the productivity of hairy root cultures is essential. The aim of this study was to optimize a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::775f86ff0a34a8223e8efbf3e85bcb5b