FAIR in action - a flexible framework to guide FAIRification

Autor: Danielle Welter, Nick Juty, Philippe Rocca-Serra, Fuqi Xu, David Henderson, Wei Gu, Jolanda Strubel, Robert T. Giessmann, Ibrahim Emam, Yojana Gadiya, Tooba Abbassi-Daloii, Ebtisam Alharbi, Alasdair J. G. Gray, Melanie Courtot, Philip Gribbon, Vassilios Ioannidis, Dorothy S. Reilly, Nick Lynch, Jan-Willem Boiten, Venkata Satagopam, Carole Goble, Susanna-Assunta Sansone, Tony Burdett

Publikováno v: Scientific Data, Vol 10, Iss 1, Pp 1-9 (2023)

Abstract The COVID-19 pandemic has highlighted the need for FAIR (Findable, Accessible, Interoperable, and Reusable) data more than any other scientific challenge to date. We developed a flexible, multi-level, domain-agnostic FAIRification framework,

Externí odkaz: https://doaj.org/article/8abc5ecd6f3c4613a806fc5ab70baccf

Recommendations for performing measurements of apparent equilibrium constants of enzyme-catalyzed reactions and for reporting the results of these measurements

Autor: Robert N. Goldberg, Robert T. Giessmann, Peter J. Halling, Carsten Kettner, Hans V. Westerhoff

Publikováno v: Beilstein Journal of Organic Chemistry, Vol 19, Iss 1, Pp 303-316 (2023)

The measurement of values of apparent equilibrium constants K′ for enzyme-catalyzed reactions involve a substantial number of critical details, neglect of which could lead to systematic errors. Here, interferences, impurities in the substances used

Externí odkaz: https://doaj.org/article/75af9735f6d14157b688b01859e67ecf

Efficient Biocatalytic Synthesis of Dihalogenated Purine Nucleoside Analogues Applying Thermodynamic Calculations

Autor: Heba Yehia, Sarah Westarp, Viola Röhrs, Felix Kaspar, Robert T. Giessmann, Hendrik F.T. Klare, Katharina Paulick, Peter Neubauer, Jens Kurreck, Anke Wagner

Publikováno v: Molecules, Vol 25, Iss 4, p 934 (2020)

The enzymatic synthesis of nucleoside analogues has been shown to be a sustainable and efficient alternative to chemical synthesis routes. In this study, dihalogenated nucleoside analogues were produced by thermostable nucleoside phosphorylases in tr

Externí odkaz: https://doaj.org/article/f740c0ac3c5f401993ed0789cad5afbd

Recommendations for performing measurements of apparent equilibrium constants of enzyme-catalyzed reactions and for reporting the results of these measurements

Autor: Carsten Kettner, Robert T. Giessmann, Peter Halling, Robert N. Goldberg, Hans Westerhoff

Publikováno v: Goldberg, R N, Giessmann, R T, Halling, P J, Kettner, C & Westerhoff, H V 2023, ' Recommendations for performing measurements of apparent equilibrium constants of enzyme-catalyzed reactions and for reporting the results of these measurements ', Beilstein Journal of Organic Chemistry, vol. 19, pp. 303-316 . https://doi.org/10.3762/bjoc.19.26
Beilstein Journal of Organic Chemistry, 19, 303-316. Beilstein-Institut Zur Forderung der Chemischen Wissenschaften

The measurement of values of apparent equilibrium constants K′ for enzyme-catalyzed reactions involve a substantial number of critical details, neglect of which could lead to systematic errors. Here, interferences, impurities in the substances used

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec43530681ec513439a2c23e407dd139
https://research.vu.nl/en/publications/7763b572-ee9c-48dd-89bb-cc0c3ae5d468

A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

Autor: Felix Kaspar, Robert T. Giessmann, Niels Krausch, Peter Neubauer, Anke Wagner, Matthias Gimpel

Publikováno v: Methods and Protocols, Vol 2, Iss 3, p 60 (2019)

Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation reactions,

Externí odkaz: https://doaj.org/article/1758685d4cb447328dd69a45f2394b9b