Zobrazeno 1 - 10
of 19
pro vyhledávání: '"T. D. Yager"'
Autor:
D. L. Sampson, B. A. Fox, T. D. Yager, S. Bhide, S. Cermelli, L. C. McHugh, T. A. Seldon, R. A. Brandon, E. Sullivan, J. J. Zimmerman, M. Noursadeghi, R. B. Brandon
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
Abstract The innate immune system of humans and other mammals responds to pathogen-associated molecular patterns (PAMPs) that are conserved across broad classes of infectious agents such as bacteria and viruses. We hypothesized that a blood-based tra
Externí odkaz:
https://doaj.org/article/828090fc6ff64bd89028a646abfae885
Autor:
P H von Hippel, T D Yager
Publikováno v:
Science. 255:809-812
At any template position, the decision to extend the transcript by one residue or to release the nascent RNA represents a kinetic competition between elongation and termination pathways. This competition is discussed in terms of alternative Eyring tr
Publikováno v:
Journal of Molecular Biology. 220:325-333
In this paper we examine the physical properties and potential for self-association of the Escherichia coli transcription factors, σ 70 and NusA. We show, by a combination of chemical crosslinking, equilibrium and velocity sedimentation, quasi-elast
Publikováno v:
Electrophoresis. 22(10)
We present a new method for the linearization and alignment of data traces generated by multilane automated DNA sequencing instruments. Application of this method to data generated with the Visible Genetics Open Gene DNA sequencing system (using Micr
Autor:
D W, Chan, T D, Yager
Publikováno v:
Chromosoma. 107(1)
We describe a method for preparing nuclear spreads from cells of live, unfixed zebrafish embryos at the late-gastrula (approximately 8000 cell) stage of development. The method consists of a sequence of four steps: (1) a slow, gentle lysis, in low to
Publikováno v:
Biochemistry and cell biology = Biochimie et biologie cellulaire. 75(5)
We describe a rapid and sensitive method for high-resolution imaging at the cellular and subcellular levels in the whole-mount zebrafish embryo. The procedure involves fixing and staining the embryo, followed by deyolking and flattening it under a co
Publikováno v:
Biochemistry. 32(1)
We show that the amino acid analogue betaine shares with small tetraalkylammonium ions [Melchior, W. B., Jr., & von Hippel, P. H. (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 298-302] the ability to reduce or even eliminate the base pair composition depe
Publikováno v:
Biochemistry. 31(1)
To function as a DNA-RNA helicase in rho-dependent transcript termination, six genetically identical subunits of the Escherichia coli transcription termination protein rho must first assemble into a hexameric complex. To help determine the quaternary
Publikováno v:
Annual review of biophysics and biomolecular structure. 21
This review has summarized the known features of the single-nucleotide addition reaction cycle in transcription. The reader will have noted that the information available is very incomplete, and that, in some cases, related experiments seem to lead t
Publikováno v:
Trends in biochemical sciences. 16(12)
The Human Genome Project (HGP) is an international effort to map and sequence the human genome. It combines skills from diverse fields of biological and technological research, thus establishing deeper interactions between scientific disciplines. The