Zobrazeno 1 - 10
of 767
pro vyhledávání: '"T-DNA Binary system"'
Publikováno v:
Plasmid. 93:24-29
Conjugative plasmids encode genes that enable them to transfer, by conjugation, from a given host cell to another cell. Conjugative transfer, despite being an important feature of conjugative plasmids, is not constitutive for most plasmids, the reaso
Publikováno v:
AIMS Microbiology. 3:784-797
Bacteria undergo horizontal gene transfer via various mechanisms. We recently reported that cell-to-cell transfer of nonconjugative plasmids occurs between strains of Escherichia coli in co-cultures, and that a specific strain (CAG18439) causes frequ
Publikováno v:
Plant cell reports. 17(6-7)
Co-transformation was investigated as a method that would allow the use of a selectable marker during plant regeneration followed by recovery of progeny which contain the desired gene(s) but lack a marker gene. Rapeseed (Brassica napus cv `212/86') a
Autor:
Satoshi Inatomi, Masumi Yoshida, Toru Okuhara, Goro Taguchi, Tomotsugu Noda, Ryohei Nishikawa, Makoto Shimosaka
Publikováno v:
Annals of Microbiology. 66:825-832
A series of plasmid vectors, named pFungiway, was constructed for the purpose of simple and versatile gene manipulation in a wide range of fungi. The backbone of these vectors consists of the binary plasmid pCAMBIA2200, which can be introduced into f
Autor:
Jian-Hua Liu, Zhenling Zeng, Lanping Liu, Xiaojie Chen, Dandan He, Wuling Liu, Sally R. Partridge, Luchao Lv
Publikováno v:
Antimicrobial Agents and Chemotherapy. 59:4464-4470
Three hybrid CTX-M β-lactamases, CTX-M-64, CTX-M-123, and CTX-M-132, with N and C termini matching CTX-M-1 group enzymes and centers matching CTX-M-9 group enzymes, have been identified. The hybrid gene sequences suggested recombination between bla
Autor:
Xinchang Shi, Junzhi Wang
Publikováno v:
Molecular Medicine Reports. 12:4669-4677
The present study aimed to develop a symbiotic selection-marker-free plasmid and host system that would allow successful plasmid maintenance and amplification for use in gene therapy. Initially, the chromosomal aspartate‑semialdehyde dehydrogenase
Publikováno v:
Biotechnology and Bioprocess Engineering. 20:396-402
An RpoD complementary system was constructed to increase the plasmid stability in Escherichia coli (E. coli). The E. coli strain carrying the rpoD285 mutation on its chromosome could not grow at high temperature without a plasmid containing the wild-
Autor:
Miguel A. Cevallos, Ofelia E. Carreón-Rodríguez, Ángeles Pérez-Oseguera, América Rivera-Urbalejo
Publikováno v:
Plasmid. 78:48-58
The maintenance of large plasmid in a wide variety of alpha-proteobacteria depends on the repABC replication/segregation unit. The intergenic repB-repC region of these plasmids encodes a countertranscribed RNA (ctRNA) that modulates the transcription
Publikováno v:
Plasmid. 93
Conjugative plasmids encode the genes responsible for the synthesis of conjugative pili and plasmid transfer. Expression of the conjugative machinery (including conjugative pili) may be costly to bacteria, not only due to the energetic/metabolic cost
Publikováno v:
Applied and Environmental Microbiology. 80:4887-4897
Several gene clusters that are responsible for toxin synthesis in bloom-forming cyanobacteria have been found to be associated with transposable elements (TEs). In particular, insertion sequence (IS) elements were shown to play a role in the inactiva