Zobrazeno 1 - 10
of 48
pro vyhledávání: '"T W, Hutchens"'
Publikováno v:
Journal of Pediatric Gastroenterology and Nutrition. 21:37-43
Lactoferrin, an iron-binding glycoprotein that is abundant in milk of some species, has been suggested to play a key role in the absorption of iron in human infants. This hypothesis is based on the dominant role of lactoferrin as an iron-binding comp
Autor:
Peter J. Reeds, T. W. Hutchens
Publikováno v:
The Journal of Nutrition. 124:1754S-1763S
Today because of tools capable of probing the details of metabolism, we are able to study the utilization of amino acids in specific, physiologically important processes. Such information may lead to recommended dietary allowances for each amino acid
Autor:
T W, Hutchens
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 11
Thiophilic adsorption is useful for the purification of immunoglobulins under mild conditions (e.g., see ref. 1). Although there are several established procedures for the purification of immunoglobulins (2-5), thiophilic adsorption appears thus far
Autor:
Judy Rosenberger, Buford L. Nichols, David L. Hachey, Gary Cook, J. S. Perkinson, T W Hutchens, Peter J. Reeds, Mary A. Dudley, A Quaroni
Publikováno v:
Journal of Biological Chemistry. 268:13609-13616
We estimated in vivo turnover rates of sucrase-isomaltase and lactase-phlorizin hydrolase in adult rats. Fed animals received a primed continuous infusion of phenylalanine (300 microCi, 150 mumol Phe/100 g of body weight for 30 s, then 7.5 microCi, 3
Autor:
T. W. Hutchens, Tai-Tung Yip
Publikováno v:
Journal of Chromatography A. 604:133-141
This preliminary investigation tests the premise that biologically relevant (1) peptide-metal ion interactions, and (2) metal ion-dependent macromolecular recognition events (e.g., peptide-peptide interactions) may be modeled by biomimetic affinity c
Autor:
T. W. Hutchens, Tai-Yung Yip
Publikováno v:
Journal of Inorganic Biochemistry. 42:105-118
We have used equilibrium binding analyses to evaluate the influence of temperature and urea on the affinity of hen egg white lysozyme and bovine pancreatic ribonuclease A for surface-immobilized Cu(II) ions. Linear Scatchard plots suggested that thes
Autor:
T. W. Hutchens, Tai-Tung Yip
Publikováno v:
Journal of Chromatography A. 536:1-15
We have evaluated immobilized Cu(II) ions as a potential site-directed molecular probe to monitor ligand-induced alterations in protein surface structures. Metal ion-induced alterations in the surface structures of different lactoferrins (human and p
Publikováno v:
Pediatric Research. 28:176-181
Although some degree of structural homology has been demonstrated among lactoferrins of different species, other reports suggest that these proteins are immunologically distinct. Human, bovine, and porcine lactoferrins were purified to homogeneity fr
Publikováno v:
Molecular Endocrinology. 4:255-267
A new antiestrogen affinity ligand for the covalent labeling of estrogen receptors, [3H]desmethylnafoxidine aziridine, has been used to investigate the salt- and temperature-independent formation of DNA-binding estrogen receptor forms from untransfor
Publikováno v:
Journal of Immunological Methods. 128:89-99
In order to evaluate the collective contribution of immunoglobulins to gastrointestinal and immune system development in newborn infants, it would be advantageous to develop a simple and rapid procedure for the selective and quantitative removal of a