Structural characterization of a PCP–R didomain from an archaeal nonribosomal peptide synthetase reveals novel interdomain interactions

Autor: J. Shaun Lott, Eric Altermann, T. Verne Lee, Vijayalekshmi Sarojini, Sandesh Deshpande

Publikováno v: The Journal of Biological Chemistry

Nonribosomal peptide synthetases (NRPSs) are multimodular enzymes that produce a wide range of bioactive peptides, such as siderophores, toxins, and antibacterial and insecticidal agents. NRPSs are dynamic proteins characterized by extensive interdom

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https://doi.org/10.1016/j.jbc.2021.100432

Prediction of the substrate for nonribosomal peptide synthetase (NRPS) adenylation domains by virtual screening

Autor: Vickery L. Arcus, Richard D. Johnson, T. Verne Lee, J. Shaun Lott

Publikováno v: Proteins: Structure, Function, and Bioinformatics. 83:2052-2066

Nonribosomal peptide synthetases (NRPSs) synthesize a diverse array of bioactive small peptides, many of which are used in medicine. There is considerable interest in predicting NRPS substrate specificity in order to facilitate investigation of the m

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https://doi.org/10.1002/prot.24922

Identification of extracellular siderophores and a related peptide from the endophytic fungus Epichloë festucae in culture and endophyte-infected Lolium perenne

Autor: Vickery L. Arcus, Susanne Rasmussen, Geoffrey A. Lane, Linda J. Johnson, Karl Fraser, Albert Koulman, J. Shaun Lott, T. Verne Lee

Publikováno v: Phytochemistry

Graphical abstract Epichloënin A (1) has been isolated from fungal cultures together with a minor variant epichloënin B, and ferriepichloënin A and a related peptide epichloëamide have been found in endophyte-infected plants. Highlights ► Epich

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https://doi.org/10.1016/j.phytochem.2011.11.020

Structure of a Eukaryotic Nonribosomal Peptide Synthetase Adenylation Domain That Activates a Large Hydroxamate Amino Acid in Siderophore Biosynthesis

Autor: Linda J. Johnson, Vickery L. Arcus, Albert Koulman, Richard D. Johnson, J. Shaun Lott, T. Verne Lee, Geoffrey A. Lane

Publikováno v: Journal of Biological Chemistry. 285:2415-2427

Nonribosomal peptide synthetases (NRPSs) are large, multidomain proteins that are involved in the biosynthesis of an array of secondary metabolites. We report the structure of the third adenylation domain from the siderophore-synthesizing NRPS, SidN,

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https://doi.org/10.1074/jbc.m109.071324

Fungal NRPS-dependent siderophores:from function to prediction

Autor: Michael Knudsen, Christian N. S. Pedersen, Teis Esben Sondergaard, T. Verne Lee, Jens Laurids Sørensen, Frederik Teilfeldt Hansen, Claus Olesen, Patricia Romans Fuertes, Ditlev E. Brodersen, Henriette Giese

Publikováno v: Sørensen, J L, Knudsen, M, T. Hansen, F, Olesen, C, Romans Fuertes, P, V. Lee, T, Søndergaard, T E, Pedersen, C S, Brodersen, D E & Giese, H 2014, Fungal NRPS-dependent siderophores : from function to prediction . in Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites . Springer Publishing Company, Fungal Biology, pp. 317-339 . https://doi.org/10.1007/978-1-4939-1191-2_15
Sørensen, J L, Knudsen, M, Hansen, F T, Olesen, C, Romans Fuertes, P, Lee, T V, Søndergaard, T E, Pedersen, C N S, Brodersen, D E & Giese, H 2014, Fungal NRPS-dependent siderophores: From function to prediction . in J-F Martin, C Garcia-Estrada & S Zeilinger (eds), Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites . Springer, Fungal Biology, pp. 317-339 . https://doi.org/10.1007/978-1-4939-1191-2_15
Fungal Biology ISBN: 9781493911905

Iron is an essential, yet often limiting element for the growth of many organisms. In response to iron limitation, fungi have developed siderophores that provide a high-affinity iron uptake system and safe intracellular storage and transport mechanis

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https://vbn.aau.dk/da/publications/2c2bbd8c-e3b4-4b13-97d7-eddab884ca21