Výsledky vyhledávání

In vivo transgenic mutation assays

Autor: J A, Heddle, S, Dean, T, Nohmi, M, Boerrigter, D, Casciano, G R, Douglas, B W, Glickman, N J, Gorelick, J C, Mirsalis, H J, Martus, T R, Skopek, V, Thybaud, K R, Tindall, N, Yajima

Publikováno v: Environmental and molecular mutagenesis. 35(3)

Transgenic rodent gene mutation models provide quick and statistically reliable assays for mutations in the DNA from any tissue. For regulatory applications, assays should be based on neutral genes, be generally available in several laboratories, and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9e02719958bc93ea898d15766add41be
https://pubmed.ncbi.nlm.nih.gov/10737959

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Culture and propagation of Hprt mutant T-lymphocytes isolated from mouse spleen

Autor: Q, Meng, T R, Skopek, D M, Walker, S, Hurley-Leslie, T, Chen, D M, Zimmer, V E, Walker

Publikováno v: Environmental and molecular mutagenesis. 32(3)

The optimization of the mouse lymphocyte Hprt mutation assay has been impeded by the relatively poor growth potential of mouse T-cells in vitro, which leads to low cloning efficiencies (CEs) and limited expansion of Hprt mutant clones for molecular a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e7e6f3a0ea73f46d31d2a0394412d0d5
https://pubmed.ncbi.nlm.nih.gov/9814438

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Transgenic mutation models: research, testing, and reality checks

Autor: T R, Skopek

Publikováno v: Environmental and molecular mutagenesis. 32(2)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::870b043cb69a3c8d008419343f0a1b67
https://pubmed.ncbi.nlm.nih.gov/9776170

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Frequency and spectrum of ethylnitrosourea-induced mutation at the hprt and lacI loci in splenic lymphocytes of exposed lacI transgenic mice

Autor: V E, Walker, N J, Gorelick, J L, Andrews, T R, Craft, J G, deBoer, B W, Glickman, T R, Skopek

Publikováno v: Cancer research. 56(20)

The development of mouse models with the endogenous hypoxanthine-guanine phosphoribosyl transferase (hprt) gene and lacI transgene as mutational targets provides an excellent opportunity to compare the mutant frequency (Mf) and types of mutations ind

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::790f7a50526d6cf3bfa56d5e4b0aa01e
https://pubmed.ncbi.nlm.nih.gov/8840980

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Germinal HPRT splice donor site mutation results in multiple RNA splicing products in T-lymphocyte cultures

Autor: Richard J. Albertini, S. Taft, T. R. Skopek, S. B. Melancon, L. Dallaire, J.P. O'Neill, T. C. Hunter

Publikováno v: Somatic cell and molecular genetics. 22(2)

We have used peripheral blood T-lymphocyte cultures to analyze the hprt mutation in two Lesch-Nyhan syndrome males who are cousins and to confirm the carrier status of female members of the family. Both cDNA and genomic DNA sequencing studies show th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e8b5f3ee9d2eda6b34134cdd117a2b4
https://pubmed.ncbi.nlm.nih.gov/8782493

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Mutagenic response of the endogenous hprt gene and lacI transgene in benzo[a]pyrene-treated Big Blue B6C3F1 mice

Autor: T R, Skopek, K L, Kort, D R, Marino, L V, Mittal, D R, Umbenhauer, G M, Laws, S P, Adams

Publikováno v: Environmental and molecular mutagenesis. 28(4)

Big Blue (BB) and generic B6C3F1 mice were given one to three i.p. injections of 50 mg/kg benzo[a]pyrene (B[a]P) in DMSO every other day to achieve cumulative doses of 50 to 150 mg/kg. Three weeks after treatment, the mutation frequency at the endoge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::779b05a598ba718acc9f1ab8b445e42c
https://pubmed.ncbi.nlm.nih.gov/8991066

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In vitro mutational spectrum of aflatoxin B1 in the human hypoxanthine guanine phosphoribosyltransferase gene

Autor: N F, Cariello, L, Cui, T R, Skopek

Publikováno v: Cancer research. 54(16)

The in vitro mutational spectrum of aflatoxin B1 (AFB1) in exon 3 of the human hypoxanthine guanine phosphoribosyltransferase gene in B-lymphoblasts was examined by a combination of polymerase chain reaction and denaturing gradient gel electrophoresi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::18337ca11c7dc8527137cfb7ca022d80
https://pubmed.ncbi.nlm.nih.gov/8044792

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International Commission for Protection Against Environmental Mutagens and Carcinogens. Working paper no. 3. Somatic mutant frequency, mutation rates and mutational spectra in the human population in vivo

Autor: J, Cole, T R, Skopek

Publikováno v: Mutation research. 304(1)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::77414957ef8e2b76135523047516c7df
https://pubmed.ncbi.nlm.nih.gov/7506357

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International Commission for Protection Against Environmental Mutagens and Carcinogens. Spontaneous mutation and its place in risk assessment for chemical mutagens. Report of an ICPEMC Committee

Autor: B A, Bridges, J, Cole, J, Favor, B W, Glickman, H, Mohrenweiser, K, Sankaranarayanan, T R, Skopek

Publikováno v: Mutation research. 304(1)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5b601fce2ba8d299b3488a5aba334127
https://pubmed.ncbi.nlm.nih.gov/7506356

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The effects of hypoxia and cysteamine on X-ray mutagenesis in human cells. II. hprt mRNA expression and cDNA sequence analysis of induced mutants

Autor: C M, Denault, T R, Skopek, H L, Liber

Publikováno v: Radiation research. 136(2)

Mutants at the hprt locus isolated after treatment of cells of the human lymphoblastoid cell line TK6 with X rays were examined by Northern blot and cDNA sequence analysis. In previous work, Southern blot analysis showed that approximately 25% of the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::12ff6714f960487f682f743c347c00bc
https://pubmed.ncbi.nlm.nih.gov/8248485

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