Zobrazeno 1 - 10
of 15
pro vyhledávání: '"T L, Gioannini"'
Publikováno v:
Neurochemical research. 24(1)
We have investigated the role of cysteine residues in a highly purified mu opioid receptor protein (muORP) by examining the effect of -SH reagents on the binding of opioid ligands. Treatment of muORP, which is devoid of additional proteins, eliminate
Autor:
G K, Ehrlich, M L, Andria, X, Zheng, B, Kieffer, T L, Gioannini, J M, Hiller, J E, Rosenkranz, B M, Veksler, R S, Zukin, E J, Simon
Publikováno v:
Canadian journal of physiology and pharmacology. 76(3)
Previous work suggested that sulfhydryl groups and disulfide bridges have important functions in opioid binding to the delta opioid receptor. The question regarding which cysteines are essential for ligand binding was approached by replacement of cys
Publikováno v:
Molecular pharmacology. 44(4)
Two peptides, which have no significant homology with known protein structures, were obtained by microsequencing of a mu-opioid binding protein purified to homogeneity from bovine striatal membranes. Polyclonal antibodies generated against portions o
Publikováno v:
Biochemical and biophysical research communications. 194(2)
An opioid binding protein (OBP) purified to homogeneity from bovine striatal membranes has been reconstituted in liposomes. The liposomes were produced by PEG-precipitation of OBP in the presence of a CHAPS extract of bovine striatum, devoid of opioi
Autor:
T. L. Gioannini, E. J. Simon
Publikováno v:
Opioids ISBN: 9783642774621
This chapter will deal primarily with what researchers have been able to learn about the properties of opioid receptors, or more precisely, of opioid binding sites, by isolating and purifying them. In order to do this with clarity it is necessary to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f87770862bb92ad77d23df69c321c9a2
https://doi.org/10.1007/978-3-642-77460-7_1
https://doi.org/10.1007/978-3-642-77460-7_1
Publikováno v:
NIDA research monograph. 126
Publikováno v:
NIDA research monograph. 119
Publikováno v:
The Journal of pharmacology and experimental therapeutics. 253(1)
In a modified Krebs buffer at 37 degrees C, the selective mu agonist [3H] D-Ala2,MePhe4,Gly-ol5]enkephalin [( 3H]DAMGO) and the nonselective mu/delta agonist human [125I]beta-endorphin [( 125I]beta-endH) bound to rat striatal membranes with a Kd of a
Publikováno v:
The Journal of biological chemistry. 260(19)
125I-beta-Endorphin (human) binds with high affinity, specificity, and saturability to rat brain and neuroblastoma X glioma hybrid cell (NG 108-15) membranes. Dissociation constants and binding capacities were obtained from Scatchard plots and are 2
Publikováno v:
Chemischer Informationsdienst. 16
Synthesis and opioid radioreceptor assay data on analogues closely related to 6-desoxy-6-spiro-alpha-methylene-gamma-lactone 5a, a compound with irreversible activity in this assay, are reported. Saturated lactones (7a,b), endocyclic alpha, beta-unsa