Výsledky vyhledávání - "T I, Ulanova"

A New Artificial Antigen of the Hepatitis E Virus

Autor: Yu. E. Khudyakov, A. N. Burkov, A. P. Obriadina, T. I. Ulanova, Howard A. Fields, G. Talekar

Publikováno v: Journal of Immunoassay and Immunochemistry. 30:18-39

An artificial antigen composed of 12 small antigenic regions derived from the ORF2 and ORF3 HEV proteins was designed. The gene encoding for this artificial antigen was assembled from synthetic oligonucleotides by a new method called Restriction Enzy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26ae0661569e9de3db2ab58bec394f60
https://doi.org/10.1080/15321810802570269

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[Effect of pH of Adsorption Buffers on the Number and Antigen-Binding Activity of Monoclonal Antibodies Immobilized on the Surface of Polystyrene Microplates]

Autor: Yu N, Tarakanova, A D, Dmitriev, Yu S, Massino, A A, Pechelulko, O L, Segal, Yu O, Skoblov, T I, Ulanova, V F, Lavrov, D A, Dmitriev

Publikováno v: Prikladnaia biokhimiia i mikrobiologiia. 51(4)

The change in the concentration and antigen-binding activity of 28 monoclonal antibodies was studied after their adsorption on the surface of polystyrene microplates in buffers with different pH values (1.0, 2.8, 7.5, 9.6, and 11.9). We used 16 clone

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::85402f0c6919b186265b759bbe38f66c
https://pubmed.ncbi.nlm.nih.gov/26353408

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[The comparative study of specificity of test-systems in diagnostic of HIV-infection on categories of samples of blood serum of pregnant women]

Autor: I N, Sharipova, N M, Khodak, V F, Puzirev, A N, Burkov, T I, Ulanova

Publikováno v: Klinicheskaia laboratornaia diagnostika. 60(3)

The detection of false positive serological reactions (FPSR) on HIV-infection under screening examination of pregnant women is an actual problem of practical health care. The original observations testify that under analysis of the same samples of bl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::15af29d5adc6999035c9fd3acfdfce2c
https://pubmed.ncbi.nlm.nih.gov/26031164

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[The DS-DIF-KORINE kit to identify microorganisms corynebacterium, including C. diphtheriae]

Autor: G A, Sorokina, N V, Zalesskikh, A P, Obriadina, T I, Ulanova, A N, Burkov, A G, Nekhorosheva, L Z, Skala, I N, Lukin

Publikováno v: Klinicheskaia laboratornaia diagnostika. (7)

The DS-DIF-KORINE kit make it possible to identify 20 types of corynebacteriae entered the Bergi identification guide (1997) including biovars gravis and mitis and belfanti version of diphtheria agent. The possibility to determine its toxigenic chara

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::16ca2f0babac5032af4ac3f5c173c01f
https://pubmed.ncbi.nlm.nih.gov/22988805

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[DNA immunization with a plasmid containing gene of hepatitis C virus protein 5A (NS5A) induces the effective cellular immune response]

Autor: O V, Masalova, E I, Lesnova, V V, Grabovetskiĭ, O A, Smirnova, T I, Ulanova, A N, Burkov, A V, Ivanov, A D, Zaberezhnyĭ, R I, Ataullakhanov, A A, Kushch

Publikováno v: Molekuliarnaia biologiia. 44(2)

In spite of extensive research, no effective vaccine against hepatitis C virus (HCV) has been developed so far. DNA immunization is a potent technique of vaccine design strongly promoting the cellular arm of immune response. The genes encoding nonstr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::59f84774ce44d5e9ce63440e4b28eea3
https://pubmed.ncbi.nlm.nih.gov/20586188

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[Use of pH-sensitive immobilized monoclonal antibodies for optimization of immunoenzyme sandwich technique of detection of HBsAg of hepatitis B virus]

Autor: D A, Iakovleva, A D, Dmitriev, D A, Dmitriev, G I, Koliaskina, Iu S, Massino, E V, Pavlova, I Iu, Pyrenkova, M B, Smirnova, O L, Segal, Iu N, Tarakanova, T I, Ulanova, O N, Fartushnaia, I N, Sharipova, V F, Lavrov

Publikováno v: Zhurnal mikrobiologii, epidemiologii i immunobiologii. (2)

To develop highly sensitive sandwich technique for identification of surface hepatitis B virus antigen (HBsAg) in serum and analyse of possible improvement of solid phase for immunoenzyme sandwich technique of HBsAg identification through variation o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::49a4714ae40193f666419576b24a0b30
https://pubmed.ncbi.nlm.nih.gov/20468098

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[HIV-1 resistance in the Yamal region and the comparison of mutation frequency with various score values]

Autor: L Iu, Volova, L A, Grezina, T I, Ulanova

Publikováno v: Klinicheskaia laboratornaia diagnostika. (2)

The amino acid sequence of the known strain HXB-2 belonging to subtype B is used as a reference strain matrix to determine HIV genotypic resistance by the sequencing technique. However, numerous studies have ascertained that HIV non-B subtypes have b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::bbe2038ceeb22c7981e9232c22819697
https://pubmed.ncbi.nlm.nih.gov/20397579

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[The capacities of current test systems to verify early HIV infection]

Autor: E N, Baranova, I N, Sharipova, N M, Denisova, M E, Susekina, V F, Puzyrev, K A, Sarkisian, M S, Vorob'eva, A N, Burkov, T I, Ulanova

Publikováno v: Voprosy virusologii. 54(5)

The purpose of the present investigation was to comparatively evaluate the performance characteristics of the test systems designed to verify the positive results of screening survey for HIV infection, such as the solid-phase immunoassay DS-EIA-HIV-A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b3822aec8c2dee0a938246de95f8b40a
https://pubmed.ncbi.nlm.nih.gov/19882902

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[Mapping of diagnostically important antigenic regions of major outer membrane protein (MOMP) from Chlamydia trachomatis using recombinant proteins with overlapping structures]

Autor: N V, Savel'eva, Iu E, Zagriadskaia, S V, Klimashevskaia, V F, Puzyrev, A N, Burkov, A P, Obriadina, T I, Ulanova

Publikováno v: Molekuliarnaia genetika, mikrobiologiia i virusologiia. (3)

Amino acid sequence of the Chlamydia trachomatis major outer membrane protein (MOMP) was modeled using a series of the recombinant proteins containing six 100 aa fragments of MOMP overlapped in 30 aa. Testing of recombinant antigens in EIA showed tha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::35f916a1e668d91a75318bca7f6fc95c
https://pubmed.ncbi.nlm.nih.gov/19705778

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[Antigenic activity of human herpes virus type 8 proteins coding orfK8.1 and orf65]

Autor: N V, Kornienko, V K, Pimenov, M V, Gladysheva, T I, Ulanova, V F, Puzyrev, A N, Burkov, A P, Obriadina

Publikováno v: Voprosy virusologii. 54(3)

A peptide scanning of the sequences of human herpes virus type 8 (HHV-8) proteins coding orfK8.1 and orf65 was made. The antigenic activity of peptides was evaluated by ELISA. The fragment 101-213 aa of orfK8.1-encoded protein was shown to consist of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2ae03899ef5e0403d82d55d97a8c289a
https://pubmed.ncbi.nlm.nih.gov/19537097

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