Zobrazeno 1 - 10
of 72
pro vyhledávání: '"T I, Bulycheva"'
Publikováno v:
Медицинская иммунология, Vol 16, Iss 5, Pp 437-442 (2014)
We have conducted a study of a novel monoclonal A3 antibody raised by means of hybridoma biotechnology. The study was performed with malignant cells of the patients with various lymphoproliferative disorders, and persons with nonmalignant diseases, a
Externí odkaz:
https://doaj.org/article/ce932ee077a94ad58176c23dd09c4f50
Publikováno v:
Bulletin of Experimental Biology and Medicine. 150:258-262
We describe the reaction of nuclei in cultured human cells from different tissues to inhibition of total protein synthesis with anisomycin - ribotoxin, which is now considered as a potential antitumor drug. It was shown that nucleoli in sensitive cel
Autor:
T. D. Volkova, N. L. Deineko, D. O. Koroev, N. M. Vladimirova, M. P. Filatova, A. V. Kamynina, Vladimir Shalgunov, E. V. Akhidova, N. V. Lobanova, Ya. S. Kim, T. I. Bulycheva, Olga M. Volpina
Publikováno v:
Scopus-Elsevier
Immunoactive fragments corresponding to the N-terminal (19-36) and C-terminal (283-294) regions of the NPM1.1 isoform of nucleophosmin and their shortened fragments were chosen and synthesized. Rabbits were immunized with free full-size peptides and
Publikováno v:
Applied Biochemistry and Microbiology. 44:256-263
Endogenous forms of the protein B23 were for the first time isolated from HeLa cell nuclei and their structural states were analyzed. It was demonstrated that incubation of HeLa cell nuclei in 10 mM Tris-HCl buffer (pH 7.4) led, not only to their swe
Publikováno v:
Cell and Tissue Biology. 2:191-199
In this work we have described the nucleolus response on inhibition of protein synthesis visualized by labeling with a new monoclonal antibody. An A3 antigen is observed in the form of numerous foci within a nucleolus and as a few foci over chromosom
Autor:
T. I. Bulycheva, Artemenko Eg, A N Pegova, Dudnik Oa, A P Shpakova, E G Gemjian, Malashenko Os, Natali N Dergunova, O V Zatsepina
Publikováno v:
Immunology Letters. 83:67-72
A novel monoclonal antibody (Mab) (called 3C9) against a major nucleolar phosphoprotein B23 was used to study B23 qualitative and quantitative alterations in phytohemagglutinin (PHA) -stimulated human peripheral blood lymphocytes in indirect immunofl
Publikováno v:
Klinicheskaia laboratornaia diagnostika. 59(7)
The article proposes new (immune cytochemical) mode of evaluation of reaction of blast-cell transformation of human lymphocytes under their stimulation with phytohemagglutinin instead of standard radiometric method using 3H-thymidine as a marker. The
Publikováno v:
Klinicheskaia laboratornaia diagnostika. (11)
The results of monitoring of qualitative content of 8 onco-antigens (CK, CA-242, CA-19-9, CA-125, REA, AFP, SCC, NSE) in serum of 414 patients are presented. The examination of 62 patients with tumors of different localization was carried our in dyna
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 44(3)
Endogenous forms of the protein B23 were for the first time isolated from HeLa cell nuclei and their structural states were analyzed. It was demonstrated that incubation of HeLa cell nuclei in 10 mM Tris-HCl buffer (pH 7.4) led, not only to their swe
Publikováno v:
Bulletin of experimental biology and medicine. 144(3)
Changes in the immunocytochemical status of the nucleoli during long-term (6–8 months) in vitro culturing of HeLa (carcinoma of the cervix uteri) cells were described using new A3 monoclonal antibodies selectively reacting with human cell nucleoli.