Zobrazeno 1 - 3
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pro vyhledávání: '"T D, Sirakova"'
Publikováno v:
FEMS microbiology letters. 59(3)
Chromosomal DNA from reference Yersinia strains was digested individually with 9 restriction endonucleases. DNA fragments were separated and analyzed by electrophoresis through agarose gels. The clearest fragment patterns were obtained when EcoRI was
Publikováno v:
FEMS microbiology letters. 59(1-2)
The low molecular mass protein antigens in PPD from M. bovis BCG were chemically oligomerized using sulfosuccinimidyl-4-(p-maleimidophenyl)-butyrate (S-SMPB) as a crosslinking agent. Protein oligomers with molecular mass over 90 kDa were obtained and
Publikováno v:
FEMS microbiology letters. 50(1-2)
Mycobacterium bovis BCG chromosomal DNA, digested with EcoR1 and HindIII, was used to construct a promoter library in Escherichia coli using the promoter probe plasmid pKO-1. DNA inserts of various sizes showed promoter activity judged by the level o