Zobrazeno 1 - 10
of 19
pro vyhledávání: '"T C, Beaman"'
Autor:
Robert E. Marquis, Philipp Gerhardt, Soon-Young Shin, H. Stuart Pankratz, Elizabeth G. Calvisi, T C Beaman
Publikováno v:
Applied and Environmental Microbiology. 60:3192-3197
Killing of bacterial spores by H 2 O 2 at elevated but sublethal temperatures and neutral pH occurred without lysis. However, with prolonged exposure or higher concentrations of the agent, secondary lytic processes caused major damage successively to
Publikováno v:
Journal of Microbiological Methods. 11:255-260
A method was developed to use an ordinary spectrophotometer for obtaining the refractive index increment of bacterial cells, enabling determination of their solids content by immersion refractometry. The results agreed well with values for cells of S
Publikováno v:
Applied and Environmental Microbiology. 56:2919-2921
Two subpopulations of Bacillus megaterium spores (1.360 and 1.355 g/ml) were obtained by density gradient centrifugation. The heavier spores had a higher thermoresistance (e.g., D 80 = 186 versus 81 min) and a higher DNA content (1.25 × 10 −14 ver
Publikováno v:
Journal of bacteriology. 174(13)
Thermograms of the exosporium-lacking dormant spores of Bacillus megaterium ATCC 33729, obtained by differential scanning calorimetry, showed three major irreversible endothermic transitions with peaks at 56, 100, and 114 degrees C and a major irreve
Publikováno v:
Infection and Immunity. 9:550-558
Hemodialysis was employed to simulate in vivo conditions for growth in mammalian blood, but without phagocytosis, by using the goat and Serratia marcescens as a host-parasite model. The blood stream was shunted surgically via prosthetic tubing from a
Publikováno v:
Journal of Bacteriology. 117:1335-1340
Conditions were defined for producing protoplasts with lysozyme and isolating the protoplast membranes from cells of Bacillus cereus T harvested late in the exponential growth phase just before sporogenesis. The membranes contained approximately 60%
Publikováno v:
FEMS Microbiology Letters. 24:47-51
Water distributed unequally within the dormant bacterial spore was quantified for the first time by use of three different lysozyme-sensitive morphotype spores of Bacillus megaterium. The extent of protoplast dehydration (27−29 g water/100 g wet pr
Autor:
Kanwal Mohan, Donald Luecke, Philipp Gerhardt, Ralph C. Belding, Ralph C. Gordon, T C Beaman, Charles E. Edmiston
Publikováno v:
Journal of Infectious Diseases. 135:51-54
A new technique of ex vivo hemodialysis culture was used to study the effects against Listeria monocytogenes of penicillin G and gentamicin administered intra-muscular, singly and in combination, in the goat. Bactericidal synergism of the combination
Publikováno v:
FEMS Microbiology Letters. 58:1-4
The low heat resistance ( D 100 = 0.554 min, z = 13.4°C) of dormant lysozyme-sensitized spores of Bacillus sphaericus 9602 was correlated with a low protoplast wet density (1.305 g/ml) equivalent to a high protoplast water content (61.0%, wet weight
Publikováno v:
Journal of Infectious Diseases. 135:42-50
A small hemodialysis culture unit was developed which can be attached to an arterial-venous shunt and worn by an animal for days. The unit consists of a blood channel separated by a membrane from a dialysate chamber in which microbial or mammalian ce