Zobrazeno 1 - 10
of 35
pro vyhledávání: '"T A, Grigliatti"'
Publikováno v:
Analytical Biochemistry. 320:88-103
Insect cells are an underexplored resource for functional G-protein-coupled receptor (GPCR) assays, despite a strong record in biochemical (binding) assays. Here we describe the use of vectors capable of creating stably transformed insect cell lines
Publikováno v:
Genome. 45:296-312
The white gene encodes an ABC-type transmembrane transporter that has a role in normal eye pigment deposition. In addition, overexpression in Drosophila leads to homosexual male courtship. Its human homologue has been implicated in cholesterol transp
Autor:
Gary Lesnicki, M. Marta Guarna, Tom A. Pfeifer, Douglas G. Kilburn, T. A. Grigliatti, Emily Kwan, Dave A. Theilmann
Publikováno v:
Protein Expression and Purification. 23:233-241
A modified Factor X protein was combined with a cellulose-binding domain tag and expressed in insect cell lines. The protein, CBDFX, was expressed and secreted into the medium. Stable, transformed Hi5 and Sf9 insect cell lines were generated and test
Publikováno v:
Genome. 42:1183-1193
The garnet gene was one of the first genes to be identified in Drosophila melanogaster. Mutations in the garnet gene affect both of the biochemically distinct types of pigments in the eye and disrupt pigmentation of other organs. As an initial step i
Autor:
J. Meredith, Y. Zhao, David A. Theilmann, T. A. Grigliatti, Dwayne D. Hegedus, H. W. Brock, John E. Phillips, Tom A. Pfeifer, Y. J. Wang
Publikováno v:
Archives of Insect Biochemistry and Physiology. 42:245-252
We have produced an active form of Schistocerca gregaria ion transport peptide (ITP) in an insect cell expression system. Transformed Drosophila Kc1 cells secreted a form of ITP into the cell culture medium that was proteolytically cleaved correctly
Autor:
Cynthia E. Shippam, Leslie G. Willis, Ian J. Forsythe, David A. Theilmann, T. A. Grigliatti, Sandra Stewart
Publikováno v:
Virology. 252(1):65-81
This study presents a detailed analysis of the acidic N-terminal region of theOrgyia pseudotsugatamulticapsid nucleopolyhedrovirus (OpMNPV) transactivator IE1. The N-terminal region of IE1 is rich in acidic amino acids and has been hypothesized to be
Autor:
Hugh W. Brock, Barry M. Honda, Vett K. Lloyd, T. A. Grigliatti, N. J. Clegg, David A. Sinclair, I. P. Whitehead, B. Wakimoto
Publikováno v:
Genome. 41:495-503
Suppressors of position-effect variegation (Su(var)s) in Drosophila melanogaster are usually studied in the presence of chromosomal rearrangements, which exhibit variegated expression of euchromatic genes moved near to, or heterochromatic genes moved
Autor:
Donna E. Hogge, Randy Mottus, Daniel Heffner, Jinko Graham, Ronald E. Reid, Joanna M. Lubieniecka, T. A. Grigliatti, Wayne Riggs
Publikováno v:
Frontiers in Genetics
Frontiers in Genetics, Vol 4 (2013)
Frontiers in Genetics, Vol 4 (2013)
Anthracyclines are very effective chemotherapeutic agents; however, their use is hampered by the treatment-induced cardiotoxicity. Genetic variants that help define patient's sensitivity to anthracyclines will greatly improve the design of optimal ch
Publikováno v:
The Canadian Entomologist. 127:49-58
The discovery of egg masses of Asian gypsy moth onboard Russian freighters in Pacific ports of North America and the capture of adult male gypsy moths in pheromone traps underscored the need for a positive identification of Asian and European gypsy m
Autor:
T. A. Grigliatti, Joanna M. Lubieniecka, Donna E. Hogge, Jie Liu, Jinko Graham, Ronald E. Reid, Wayne Riggs, Daniel Heffner
Publikováno v:
Cancer epidemiology, biomarkersprevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. 21(11)
Background: Evidence suggests that interpatient variability in anthracycline metabolic rate may contribute to the cardiotoxicity associated with anthracycline-based chemotherapy. Therefore, polymorphisms in the anthracycline metabolizing enzymes have