Zobrazeno 1 - 10
of 537
pro vyhledávání: '"T, Wittwer"'
Autor:
Sri Priya Ponnapalli, Matthew W. Bradley, Karen Devine, Jay Bowen, Sara E. Coppens, Kristen M. Leraas, Brett A. Milash, Fuqiang Li, Huijuan Luo, Shi Qiu, Kui Wu, Huanming Yang, Carl T. Wittwer, Cheryl A. Palmer, Randy L. Jensen, Julie M. Gastier-Foster, Heidi A. Hanson, Jill S. Barnholtz-Sloan, Orly Alter
Publikováno v:
APL Bioengineering, Vol 4, Iss 2, Pp 026106-026106-14 (2020)
Modeling of genomic profiles from the Cancer Genome Atlas (TCGA) by using recently developed mathematical frameworks has associated a genome-wide pattern of DNA copy-number alterations with a shorter, roughly one-year, median survival time in gliobla
Externí odkaz:
https://doaj.org/article/8bb5905037b540a0896d241d4848ca91
Autor:
Carl T. Wittwer
Publikováno v:
Methods in Molecular Biology ISBN: 9781071629499
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5625f2cfe6a0ae000b490698d1d71b38
https://doi.org/10.1007/978-1-0716-2950-5_14
https://doi.org/10.1007/978-1-0716-2950-5_14
Publikováno v:
BioTechniques, Vol 62, Iss 1, p xxv (2017)
Protocol Summary We have combined allele-specific PCR, competitive probe blocking, asymmetric PCR, and melting analysis to enhance rare allele detection in a homogeneous system. Unlabeled, dual hybridization or molecular beacon probes were used for c
Externí odkaz:
https://doaj.org/article/c1782ac5c2bb453e92d7135817a7d4d7
Publikováno v:
Nucleic Acids Research
We used stopped-flow to monitor hypochromicity for 43 oligonucleotide duplexes to study nucleic acid kinetics and extract transition-state parameters for association and dissociation. Reactions were performed in 1.0 M NaCl (for literature comparisons
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ee955802a505fb1d8e4448405b2f191
https://doi.org/10.1093/nar/gkab205
https://doi.org/10.1093/nar/gkab205
Publikováno v:
BioTechniques, Vol 54, Iss 6, Pp 314-320 (2013)
Rapid cycle DNA amplification was continuously monitored by three different fluorescence techniques. Fluorescence was monitored by (i) the double-strand-specific dye SYBR Green I, (ii) a decrease in fluorescein quenching by rhodamine after exonucleas
Externí odkaz:
https://doaj.org/article/29c5712a57c44720aec9857606f9d9ff
Publikováno v:
BioTechniques, Vol 50, Iss 5, Pp 311-318 (2011)
Differential amplification of variant and wild-type alleles by PCR is often used for rare allele enrichment. We have combined allele-specific PCR, competitive probe blocking, asymmetric PCR, and melting analysis to enhance rare allele detection in a
Externí odkaz:
https://doaj.org/article/e327c873936f4a979180db60043ff355
Publikováno v:
BioTechniques, Vol 44, Iss 4, Pp 487-492 (2008)
Rapid-cycle PCR uses fast temperature transitions and minimal denaturation and annealing times of “0” s to complete 30 cycles in 10 to 30 min. The most popular platform amplifies samples in glass capillaries arranged around a carousel with circul
Externí odkaz:
https://doaj.org/article/4eb2f967346a43998d90b547a09771e9
Autor:
Matthew Dean Poulson, Carl T. Wittwer
Publikováno v:
BioTechniques, Vol 43, Iss 1, Pp 87-91 (2007)
Isolated-probe PCR (IP-PCR) is a method that combines asymmetric PCR, unlabeled probes, and high-resolution DNA melting while maintaining a closed tube system. A double-stranded DNA (dsDNA) dye LCGreen® I was used to detect the unlabeled probes. LCG
Externí odkaz:
https://doaj.org/article/208d94576d4b4d92bf68d9de6efde5db
Publikováno v:
BioTechniques, Vol 39, Iss 5, Pp 644-650 (2005)
Externí odkaz:
https://doaj.org/article/e74390b1d3b1406cac277f71d69818b9
Publikováno v:
BioTechniques, Vol 29, Iss 5, Pp 1006-1012 (2000)
An alternative method of rapid-cycle PCR for DNA amplification is demonstrated using electrolyte resistance for heating and temperature monitoring. The PCR amplification solution is electrically conductive and can be heated by passing an alternating
Externí odkaz:
https://doaj.org/article/e35d506eb05448f3be8ebd329d5eae3c