Roles of multiple acyl-CoA oxidases in the routing of carbon flow towards β-oxidation and polyhydroxyalkanoate biosynthesis in Yarrowia lipolytica

Autor: Ramdane Haddouche, Syndie Delessert, Julia S. Sabirova, Cécile Neuvéglise, Yves Poirier, Jean-Marc Nicaud

Publikováno v: FEMS Yeast Research. 10:917-927

The oleaginous yeast Yarrowia lipolytica possesses six acyl-CoA oxidase (Aox) isoenzymes encoded by genes POX1-POX6. The respective roles of these multiple Aox isoenzymes were studied in recombinant Y. lipolytica strains that express heterologous pol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4d2b6a3a470950f972d4472d38a679b7
https://doi.org/10.1111/j.1567-1364.2010.00670.x

Analysis of the β-oxidation of trans-unsaturated fatty acid in recombinant Saccharomyces cerevisiae expressing a peroxisomal PHA synthase reveals the involvement of a reductase-dependent pathway

Autor: Yves Poirier, Silvia Marchesini, Julien Robert, Syndie Delessert

Publikováno v: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1734:169-177

The degradation of fatty acids having cis- or trans-unsaturated bond at an even carbon was analyzed in Saccharomyces cerevisiae by monitoring polyhydroxyalkanoate production in the peroxisome. Polyhydroxyalkanaote is synthesized by the polymerization

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12dfa0632aaf36f7f3407410f210ee4a
https://doi.org/10.1016/j.bbalip.2005.02.010

Engineering polyhydroxyalkanoate content and monomer composition in the oleaginous yeast Yarrowia lipolytica by modifying the beta-oxidation multifunctional protein

Autor: Yves Poirier, Julia S. Sabirova, Ramdane Haddouche, Syndie Delessert, Yves Pagot, Jean-Marc Nicaud, Cécile Neuvéglise

Publikováno v: Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, Springer Verlag, 2011, 91 (5), pp.1327-1340. ⟨10.1007/s00253-011-3331-2⟩

Recombinant strains of the oleaginous yeast Yarrowia lipolytica expressing the PHA synthase gene (PhaC) from Pseudomonas aeruginosa in the peroxisome were found able to produce polyhydroxyalkanoates (PHA). PHA production yield, but not the monomer co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::284dab83854dd4ec286e61bb756c7e47
https://hal.archives-ouvertes.fr/hal-01004574

Peroxisomal Delta(3),Delta(2)-enoyl CoA isomerases and evolution of cytosolic paralogues in embryophytes

Autor: Yves Poirier, Christian Tellgren-Roth, J. Kalervo Hiltunen, Charles Vidoudez, Syndie Delessert, Simon Goepfert

Publikováno v: The Plant journal : for cell and molecular biology. 56(5)

Delta(3),Delta(2)-enoyl CoA isomerase (ECI) is an enzyme that participates in the degradation of unsaturated fatty acids through the beta-oxidation cycle. Three genes encoding Delta(3),Delta(2)-enoyl CoA isomerases and named AtECI1, AtECI2 and AtECI3

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90e44340ab741e62d2ed9f4a0fc5d634
https://pubmed.ncbi.nlm.nih.gov/18657232

The peroxisomal Acyl-CoA thioesterase Pte1p from Saccharomyces cerevisiae is required for efficient degradation of short straight chain and branched chain fatty acids

Autor: Seiko Hasegawa, Yves Poirier, Syndie Delessert, Isamu Maeda, Sophie Zuber, Yoshiaki Seto

Publikováno v: The Journal of biological chemistry. 281(17)

The role of the Saccharomyces cerevisae peroxisomal acyl-coenzyme A (acyl-CoA) thioesterase (Pte1p) in fatty acid beta-oxidation was studied by analyzing the in vitro kinetic activity of the purified protein as well as by measuring the carbon flux th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8448672f50195f4ece3080505a476680
https://pubmed.ncbi.nlm.nih.gov/16490786