Zobrazeno 1 - 10
of 55
pro vyhledávání: '"Sydney A. Shain"'
Autor:
Tomo Š arić, Sydney A. Shain
Publikováno v:
BioTechniques, Vol 22, Iss 4, Pp 630-636 (1997)
Externí odkaz:
https://doaj.org/article/ff26656beeb141ae8e1dd307ebb8e4cf
Autor:
Sydney A. Shain
Publikováno v:
Molecular Cancer Research. 2:653-661
Understanding processes regulating prostate cancer cell survival is critical to management of advanced disease. We used prostate cancer cell transfectants genetically modified to be deficient in either endogenous fibroblast growth factor (FGF-1) or e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::acdc53a3c768352998741022c5a77193
https://doi.org/10.1158/1541-7786.653.2.11
https://doi.org/10.1158/1541-7786.653.2.11
Autor:
Sydney A. Shain
Publikováno v:
Molecular Urology. 5:121-130
We used rat prostate cancer cell stable transfectants that lacked either endogenous fibroblast growth factor (FGF)-1 secondary to constitutive expression of FGF-1 antisense RNA (aFa2-transfectants) or endogenous FGF-2 isoforms secondary to constituti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a98fd0a544d17986d95d4d82e253a8a6
https://doi.org/10.1089/10915360152559602
https://doi.org/10.1089/10915360152559602
Autor:
Sydney A. Shain, Tomo Saric
Publikováno v:
BioTechniques. 22:630-636
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da01b19466cc965cdef198b23c0db6b6
https://doi.org/10.2144/97224bm12
https://doi.org/10.2144/97224bm12
Publikováno v:
Protein Expression and Purification. 3:497-507
We describe the preparation of vectors for T7 polymerase-driven, high-level expression of rat acidic (aFGF) or basic (bFGF) fibroblast growth factors in Escherichia coli . Following isopropyl-β- d -thiogalacto-side induction of T7 polymerase, rat aF
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4313cfa219b1be171ebb7528da5e8c8
https://doi.org/10.1016/1046-5928(92)90067-7
https://doi.org/10.1016/1046-5928(92)90067-7
Publikováno v:
The Journal of Steroid Biochemistry and Molecular Biology. 37:85-95
Using biochemical methods we established that estrogen receptor content and distribution and progesterone receptor content in female and male baboon myocardium did not differ between sexes. In contrast, myocardial androgen receptor distribution betwe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03b273f7df824c789335d4ed08ee9436
https://doi.org/10.1016/0960-0760(90)90376-v
https://doi.org/10.1016/0960-0760(90)90376-v
Publikováno v:
Endocrinology. 126:818-825
Clonally derived C-, D-, and T-family AXC/SSh rat prostate cancer cell lines contain transforming growth factor-beta (TGF beta) receptors. The content in C3, D1, T1, and T5 cells, respectively, was 8,560 +/- 1,450, 13,160 +/- 1,240, 2,425 +/- 490, an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8ae59ac647276c81ccebbf5f2e5f6d4
https://doi.org/10.1210/endo-126-2-818
https://doi.org/10.1210/endo-126-2-818
Autor:
Sydney A. Shain, Tomo Saric
Publikováno v:
Growth factors (Chur, Switzerland). 16(1)
Using quantitative RT-PCR, we found that T1 rat prostate cancer cell relative FGF-1 transcript content was about 180-fold greater than that of FGF-2. This difference in transcript content was not representative of T1 cell relative FGF-1 and FGF-2 pro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbc83fb409a962f16070ab6fc999822e
https://pubmed.ncbi.nlm.nih.gov/9777371
https://pubmed.ncbi.nlm.nih.gov/9777371
Autor:
Alan L. Lin, Sydney A. Shain
Publikováno v:
Arteriosclerosis: An Official Journal of the American Heart Association, Inc.. 5:668-677
We used either the synthetic estrogen R2858 (moxestrol) or estradiol-17 beta to characterize estrogen receptors in cytoplasmic (R2858) and nuclear (estradiol-17 beta) preparations from rat aorta and myocardium. Relative steroid specificity studies sh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0785ff8abde5369338f073209b286e9a
https://doi.org/10.1161/01.atv.5.6.668
https://doi.org/10.1161/01.atv.5.6.668
Autor:
Alan L. Lin, Sydney A. Shain
Publikováno v:
Molecular and Cellular Endocrinology. 63:75-83
Using methods for cell lysis and fractionation which yield essentially quantitative recovery of rat prostate cancer cell cytosolic and nuclear androgen receptors, we examined androgen modulation of androgen receptor content of clonally derived prosta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1489d8123902075b9b0e15e85ede2143
https://doi.org/10.1016/0303-7207(89)90083-x
https://doi.org/10.1016/0303-7207(89)90083-x