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Autor:
Sylvia Wagner, Kenji Takabayashi, Janine Kessi, Mark D. Erion, Harry B. Smith, Spencer L. Shames, Sybille Hönger, Steven E. Ealick
Publikováno v:
Biochemistry. 36:11725-11734
To probe the catalytic mechanism of human purine nucleoside phosphorylase (PNP), 13 active-site mutants were constructed and characterized by steady-state kinetics. In addition, microtiter plate assays were developed for both the phosphorolytic and s