Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Syam P. Anand"'
Autor:
Karen R. Thickman, Sanford H. Leuba, Grant D. Schauer, Saleem A. Khan, Matt V. Fagerburg, Syam P. Anand, Piero R. Bianco
Publikováno v:
Nucleic Acids Research
The essential DNA helicase, PcrA, regulates recombination by displacing the recombinase RecA from the DNA. The nucleotide-bound state of RecA determines the stability of its nucleoprotein filaments. Using single-molecule fluorescence approaches, we d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::170e134fc2bb9d63ae8d457ec44984be
https://doi.org/10.1093/nar/gks641
https://doi.org/10.1093/nar/gks641
Publikováno v:
Journal of Bacteriology. 191:2493-2500
Bacillus anthracis contains two megaplasmids, pXO1 and pXO2, that are critical for its pathogenesis. Stable inheritance of pXO1 in B. anthracis is dependent upon the tubulin/FtsZ-like RepX protein encoded by this plasmid. Previously, we have shown th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69a2a69c51fd0b13788a1cffdaabf3b0
https://doi.org/10.1128/jb.00027-09
https://doi.org/10.1128/jb.00027-09
Publikováno v:
Chromosome Research. 16:451-467
Many questions in molecular and cellular biology can be reduced to questions about 'who talks to whom, when and how frequently'. Here, we review approaches we have used with single-pair fluorescence resonance energy transfer (spFRET) to follow the mo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6166287e8f41795164c2ddac105b9737
https://doi.org/10.1007/s10577-008-1235-5
https://doi.org/10.1007/s10577-008-1235-5
Publikováno v:
Journal of Bacteriology. 189:4502-4509
PcrA is a conserved DNA helicase present in all gram-positive bacteria. Bacteria lacking PcrA show high levels of recombination. Lethality induced by PcrA depletion can be overcome by suppressor mutations in the recombination genes recFOR . RecFOR pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cff2d92ed6031aedfc9d093316cc316
https://doi.org/10.1128/jb.00376-07
https://doi.org/10.1128/jb.00376-07
Publikováno v:
Journal of Bacteriology. 188:7416-7425
PcrA is a chromosomally encoded DNA helicase of gram-positive bacteria involved in replication of rolling circle replicating plasmids. Efficient interaction between PcrA and the plasmid-encoded replication initiator (Rep) protein is considered a requ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2705c7bb6b2790d5a4e2dbe3ac041fd
https://doi.org/10.1128/jb.01010-06
https://doi.org/10.1128/jb.01010-06
Publikováno v:
Journal of Bacteriology. 186:2195-2199
Replication of rolling-circle replicating (RCR) plasmids in gram-positive bacteria requires the unwinding of initiator protein-nicked plasmid DNA by the PcrA helicase. In this report, we demonstrate that heterologous PcrA helicases from Bacillus anth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf8e7705c82eed2aa778f0cba008393d
https://doi.org/10.1128/jb.186.7.2195-2199.2004
https://doi.org/10.1128/jb.186.7.2195-2199.2004
Publikováno v:
Journal of Biological Chemistry. 277:45880-45886
Previous genetic studies have suggested that a putative chromosome-encoded helicase, PcrA, is required for the rolling circle replication of plasmid pT181 in Staphylococcus aureus. We have overexpressed and purified the staphylococcal PcrA protein an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::028fd0d8c60472110b67179ea40d103b
https://doi.org/10.1074/jbc.m207383200
https://doi.org/10.1074/jbc.m207383200
Autor:
Syam P. Anand, Saleem A. Khan
Bacterial plasmids are extrachromosomal genetic elements having copy numbers ranging from one to a few thousand. The maintenance of plasmids in bacterial cells is dependent upon factors and processes that control their replication, selection, copy nu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1586481d57c508b66f287e017857500c
https://europepmc.org/articles/PMC2820855/
https://europepmc.org/articles/PMC2820855/
Publikováno v:
Molecular microbiology. 67(4)
RepX protein encoded by the pXO1 plasmid of Bacillus anthracis is required for plasmid replication. RepX harbours the tubulin signature motif and contains limited amino acid sequence homology to the bacterial cell division protein FtsZ. Although repl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bd67d229493d51fb2f7c5ebb9794f523
https://pubmed.ncbi.nlm.nih.gov/18179418
https://pubmed.ncbi.nlm.nih.gov/18179418
Publikováno v:
Plasmid. 54(2)
Plasmid rolling-circle replication initiates by covalent extension of a nick generated at the plasmid double-strand origin (dso) by the initiator protein. The RepC initiator protein binds to the plasmid pT181 dso in a sequence-specific manner and rec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ade75a65c092e3ae5eaf58167ca9daa
https://pubmed.ncbi.nlm.nih.gov/16122559
https://pubmed.ncbi.nlm.nih.gov/16122559