Deletion Mutants, Archived Transposon Library, and Tagged Protein Constructs of the Model Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough

Autor: Maxim Shatsky, Jayashree Ray, Thomas R. Juba, Grant M. Zane, Jennifer V. Kuehl, Kara B. De León, Kimberly L. Keller, Adam P. Arkin, Gareth Butland, Kelly S. Bender, Swapnil R. Chhabra, Adam M. Deutschbauer, Judy D. Wall, Valentine V. Trotter

Publikováno v: Microbiology resource announcements, vol 10, iss 11
Microbiology Resource Announcements

The dissimilatory sulfate-reducing deltaproteobacterium Desulfovibrio vulgaris Hildenborough (ATCC 29579) was chosen by the research collaboration ENIGMA to explore tools and protocols for bringing this anaerobe to model status. Here, we describe a c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f513b43a3458c8f94a6bc81a62b2e505
https://escholarship.org/uc/item/4zk7904n

Bacterial Interactomes: Interacting Protein Partners Share Similar Function and Are Validated in Independent Assays More Frequently Than Previously Reported

Autor: Nancy L. Liu, Sonia A. Reveco, Jil T. Geller, John-Marc Chandonia, Mary E. Singer, Whenhong Yang, Susan J. Fisher, Gareth Butland, Judy D. Wall, Bonita R. Lam, Ramadevi Prathapam, Mark D. Biggin, Steven E. Brenner, Haichuan Liu, Dwayne A. Elias, Terry C. Hazen, Barbara Gold, Jennifer He, Valentine V. Trotter, Avneesh Saini, Simon Allen, Evelin D. Szakal, Swapnil R. Chhabra, Thomas R. Juba, Steven C. Hall, H. Ewa Witkowska, Maxim Shatsky

Publikováno v: Molecular & cellular proteomics : MCP, vol 15, iss 5
Shatsky, M; Allen, S; Gold, BL; Liu, NL; Juba, TR; Reveco, SA; et al.(2016). Bacterial interactomes: Interacting protein partners share similar function and are validated in independent assays more frequently than previously reported. Molecular and Cellular Proteomics, 15(5), 1539-1555. doi: 10.1074/mcp.M115.054692. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/3z82f5ct

© 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Numerous affinity purification-mass spectrometry (APMS) and yeast two-hybrid screens have each defined thousands of pairwise protein-protein interactions (PPIs), most of whi

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https://escholarship.org/uc/item/3z82f5ct

Generalized Schemes for High-Throughput Manipulation of the Desulfovibrio vulgaris Genome

Autor: Evelin D. Szakal, Simon Allen, Manfred Auer, Swapnil R. Chhabra, Mario Ouellet, Gareth Butland, Judy D. Wall, Jay D. Keasling, Harry R. Beller, Barbara Gold, Mark D. Biggin, R. Prathapam, H. E. Witkowska, Thomas R. Juba, Sonia A. Reveco, John-Marc Chandonia, Amita Gorur, Kimberly L. Keller, C. M. Leung, Terry C. Hazen, O.-Y. Fok, Danielle M. Jorgens, Grant M. Zane, Elizabeth S. Semkiw, Adam P. Arkin, Morgan N. Price, Mary E. Singer, Dwayne A. Elias

Publikováno v: Applied and Environmental Microbiology. 77:7595-7604

The ability to conduct advanced functional genomic studies of the thousands of sequenced bacteria has been hampered by the lack of available tools for making high-throughput chromosomal manipulations in a systematic manner that can be applied across

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::644e9a3a972cb8bbb93431b3fe1e95e1
https://doi.org/10.1128/aem.05495-11

Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima

Autor: Zhiwei Chen, Kenneth L. Sale, Rajat Sapra, Ryan P. McAndrew, Blake A. Simmons, Swapnil R. Chhabra, Jose Henrique Pereira, Paul D. Adams

Publikováno v: Journal of Structural Biology. 172:372-379

Tm_Cel5A, which belongs to family 5 of the glycoside hydrolases, is an extremely stable enzyme among the endo-acting glycosidases present in the hyperthermophilic organism Thermotoga maritima. Members of GH5 family shows a common (β/α)(8) TIM-barre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dde958fdc20fae09b1f741854297791
https://doi.org/10.1016/j.jsb.2010.06.018

Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough

Autor: Terry C. Hazen, Zhili He, Qiang He, Anup K. Singh, Jizhong Zhou, Judy D. Wall, Katherine H. Huang, Eric J. Alm, Sara P. Gaucher, Masood Z. Hadi, Swapnil R. Chhabra, Adam P. Arkin

Publikováno v: Journal of Bacteriology. 188:1817-1828

Desulfovibrio vulgaris Hildenborough belongs to a class of sulfate-reducing bacteria (SRB) and is found ubiquitously in nature. Given the importance of SRB-mediated reduction for bioremediation of metal ion contaminants, ongoing research on D. vulgar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc69f7a28ca39b4b9503f2077c5933ff
https://doi.org/10.1128/jb.188.5.1817-1828.2006