A rapid molecular approach for chromosomal phasing.

Autor: John F Regan, Nolan Kamitaki, Tina Legler, Samantha Cooper, Niels Klitgord, George Karlin-Neumann, Catherine Wong, Shawn Hodges, Ryan Koehler, Svilen Tzonev, Steven A McCarroll

Publikováno v: PLoS ONE, Vol 10, Iss 3, p e0118270 (2015)

Determining the chromosomal phase of pairs of sequence variants - the arrangement of specific alleles as haplotypes - is a routine challenge in molecular genetics. Here we describe Drop-Phase, a molecular method for quickly ascertaining the phase of

Externí odkaz: https://doaj.org/article/7b37bf289b1f43f0aa67cdad56127cdc

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant

Autor: Julia Beck, Simon Cowen, Anne Pradines, Lao H. Saal, Elliot Stieglitz, Lisa Davis, Silvia Galbiati, Alexandra Pender, Tim Forshew, Filip Janku, Roger Lacave, Svilen Tzonev, Charlotte Proudhon, Valérie Combaret, Jim F. Huggett, Gerwyn M. Jones, Ana Fernandez-Gonzalez, Justin Lee, Bryan C. Ulrich, Alexandra S. Whale, Helen C. Parkes, Nicholas Redshaw, Carole A. Foy, Alison S. Devonshire, Frederic Fina, Rikke Fredslund Andersen, Andreas Berger, Vilas Mistry, Leanne Javier, George Karlin-Neumann, John F. Regan, Álvaro González Hernández, Nina Dahl Kjersgaard, Charles A. Haynes

Publikováno v: Whale, A S, Devonshire, A S, Karlin-Neumann, G, Regan, J, Javier, L, Cowen, S, Fernandez-Gonzalez, A, Jones, G M, Redshaw, N, Beck, J, Berger, A W, Combaret, V, Kjersgaard, N D, Davis, L, Fina, F, Forshew, T, Andersen, R F, Galbiati, S, Hernandez, A G, Haynes, C A, Janku, F, Lacave, R, Lee, J, Mistry, V, Pender, A, Pradines, A, Proudhon, C, Saal, L H, Stieglitz, E, Ulrich, B, Foy, C A, Parkes, H, Tzonev, S & Huggett, J F 2017, ' International interlaboratory digital PCR study demonstrating high reproducibility for the measurement of a rare sequence variant ', Analytical Chemistry, vol. 89, no. 3, pp. 1724-1733 . https://doi.org/10.1021/acs.analchem.6b03980

This study tested the claim that digital PCR (dPCR) can offer highly reproducible quantitative measurements in disparate laboratories. Twenty-one laboratories measured four blinded samples containing different quantities of a KRAS fragment encoding G

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebc01d24aa5634e6fdf1096bd5afb8ec
https://doi.org/10.1021/acs.analchem.6b03980

Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies–What Does It Mean?

Autor: Svilen Tzonev

Publikováno v: Methods in Molecular Biology ISBN: 9781493977765

Current commercially available digital PCR (dPCR) systems and assays are capable of detecting individual target molecules with considerable reliability. As tests are developed and validated for use on clinical samples, the need to understand and deve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fbc5c42cecb2558367d52c8979bc73ea
https://doi.org/10.1007/978-1-4939-7778-9_3

Variability and Information in a Neural Code of the Cat Lateral Geniculate Nucleus

Autor: Robert C. Liu, Sergei P. Rebrik, Svilen Tzonev, Kenneth D. Miller

Publikováno v: Journal of Neurophysiology. 86:2789-2806

A central theme in neural coding concerns the role of response variability and noise in determining the information transmission of neurons. This issue was investigated in single cells of the lateral geniculate nucleus of barbiturate-anesthetized cat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5c2317d110cbeac3fc0d547914dd3cd
https://doi.org/10.1152/jn.2001.86.6.2789

Droplet Digital™ PCR: Multiplex Detection of KRAS Mutations in Formalin-Fixed, Paraffin-Embedded Colorectal Cancer Samples

Autor: Jennifer R. Berman, Eli Hefner, Samantha Cooper, Bin Zhang, Svilen Tzonev, Wei Yang, John F. Regan, Dawne N. Shelton

Publikováno v: BioTechniques. 58:205-206

Targeted therapies in many cancers have allowed unprecedented progress in the treatment of disease. However, routine implementation of genomic testing is constrained due to: 1) limited amounts of sample (pg–ng range) per biological specimen, 2) dia

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06702848c1de513beaacb8e755655a5d
https://doi.org/10.2144/000114279