Eliminating Factor H-Binding Activity of Borrelia burgdorferi CspZ Combined with Virus-Like Particle Conjugation Enhances Its Efficacy as a Lyme Disease Vaccine

Autor: Ashley L. Marcinkiewicz, Ilva Lieknina, Svetlana Kotelovica, Xiuli Yang, Peter Kraiczy, Utpal Pal, Yi-Pin Lin, Kaspars Tars

Publikováno v: Frontiers in Immunology, Vol 9 (2018)

The spirochete Borrelia burgdorferi is the causative agent of Lyme disease, the most common tick-borne disease in the US and Europe. No potent human vaccine is currently available. The innate immune complement system is vital to host defense against

Externí odkaz: https://doaj.org/article/dfa1703518d14767a0216731dc6e1cde

Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning

Autor: Olivier Ouari, Diane Cala de Paepe, Inara Akopjana, Svetlana Kotelovica, G. Pintacuda, Anne Lesage, Andrea Pica, Andrew J. Pell, Kristaps Jaudzems, Andrea Bertarello, Sachin Rama Chaudhari, Emeline Barbet-Massin, Kaspars Tars, David Gajan

Publikováno v: Angewandte Chemie International Edition
Angewandte Chemie International Edition, Wiley-VCH Verlag, 2018, 57 (25), pp.7458-7462. ⟨10.1002/anie.201801016⟩
Angewandte Chemie International Edition, 2018, 57 (25), pp.7458-7462. ⟨10.1002/anie.201801016⟩

International audience; Dynamic nuclear polarization (DNP) is a powerful way to overcome the sensitivity limitation of magic-angle-spinning (MAS) NMR experiments. However, the resolution of the DNPNMR spectra of proteins is compromised by severe line

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cc9e0d62449bc45746e65fcdc1af98e
https://hal.archives-ouvertes.fr/hal-01859534

Yeast-Expressed Bacteriophage-Like Particles for the Packaging of Nanomaterials

Autor: Janis Freivalds, Tatyana Voronkova, Velta Ose, Andris Kazaks, Svetlana Kotelovica, Kaspars Tars

Publikováno v: Molecular Biotechnology. 56:102-110

Virus-like particles (VLPs) generated by heterologous expression of viral structural genes have become powerful tools in vaccine development. Recently, we and others have reported on the assembly of VLPs of the RNA bacteriophages MS2, Qβ, and GA in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2691088ad7f6a5b7d1bb226518710d16
https://doi.org/10.1007/s12033-013-9686-0

Structure of AP205 Coat Protein Reveals Circular Permutation in ssRNA Bacteriophages

Autor: Andris Kazaks, Loren B. Andreas, Christoph A. Diebolder, Inara Akopjana, Kristaps Jaudzems, Janis Rumnieks, Jan Stanek, Svetlana Kotelovica, Mihails Shishovs, Kaspars Tars, Guido Pintacuda, Roman I. Koning

Publikováno v: Journal of Molecular Biology, 428(21), 4267-4279
Journal of Molecular Biology
Journal of Molecular Biology, Elsevier, 2016, 428 (21), pp.4267-4279. ⟨10.1016/j.jmb.2016.08.025⟩

We are thankful to the MAX-lab staff for their support during our visit at the synchrotron.; International audience; AP205 is a single-stranded RNA bacteriophage that has a coat protein sequence not similar to any other known single-stranded RNA phag

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3acecd1cf5128f7d9e795a6058e31ee0
https://pubmed.ncbi.nlm.nih.gov/27591890

Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning

Autor: Victoria A. Higman, Guido Pintacuda, Loren B. Andreas, I. Akopjana, Andrew J. Pell, Anne Lesage, Monica Stoppini, Lyndon Emsley, Michele Felletti, Martino Bolognesi, Kaspars Tars, Paul Guerry, Hartmut Oschkinat, Emeline Barbet-Massin, Tanguy Le Marchand, Andrea Bertarello, Robert G. Griffin, Matthias Hiller, Vittorio Bellotti, Stefano Ricagno, Torsten Herrmann, Svetlana Kotelovica, James J. Chou, Andrew J. Nieuwkoop, J. Retel, Kristaps Jaudzems, W. Trent Franks, Michael J. Knight

Publikováno v: Journal of the American Chemical Society, 136(35): 12489-12497
Journal of the American Chemical Society
Journal of the American Chemical Society, American Chemical Society, 2014, 136 (35), pp.12489-12497. ⟨10.1021/ja507382j⟩

International audience; Using a set of six H-1-detected triple-resonance NMR experiments, we establish a method for sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of 5-30 kDa pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78e4252411a8e7063da8aae83385b123
https://repository.publisso.de/resource/frl:6405951