Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Sushmitha Paulraj"'
Autor:
Greta Bigelyte, Joshua K. Young, Tautvydas Karvelis, Karolina Budre, Rimante Zedaveinyte, Vesna Djukanovic, Elizabeth Van Ginkel, Sushmitha Paulraj, Stephen Gasior, Spencer Jones, Lanie Feigenbutz, Grace St. Clair, Pierluigi Barone, Jennifer Bohn, Ananta Acharya, Gina Zastrow-Hayes, Selgar Henkel-Heinecke, Arunas Silanskas, Ralf Seidel, Virginijus Siksnys
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
Miniature Cas12f editing systems are well suited for in vivo editing applications. Here the authors characterize the intrinsic activity of SpCas12f1 in plant and animal cells.
Externí odkaz:
https://doaj.org/article/688a63ea2c9f45e084a40d762048f444
Autor:
Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis, Darius Kazlauskas, Tomas Urbaitis, Monika Jasnauskaite, Mantvyda M. Grusyte, Sushmitha Paulraj, Po-Hao Wang, Zhenglin Hou, Shane K. Dooley, Mark Cigan, Clara Alarcon, N. Doane Chilcoat, Greta Bigelyte, Jennifer L. Curcuru, Megumu Mabuchi, Zhiyi Sun, Ryan T. Fuchs, Ezra Schildkraut, Peter R. Weigele, William E. Jack, G. Brett Robb, Česlovas Venclovas, Virginijus Siksnys
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available to
Externí odkaz:
https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c
Autor:
Tomas Urbaitis, Giedrius Gasiunas, Joshua K Young, Zhenglin Hou, Sushmitha Paulraj, Egle Godliauskaite, Mantvyda M Juskeviciene, Migle Stitilyte, Monika Jasnauskaite, Megumu Mabuchi, G Brett Robb, Virginijus Siksnys
Publikováno v:
EMBO reports, Hoboken : Wiley, 2022, vol. 23, iss. 12, art. no. e55481, p. [1-15]
Most CRISPR-type V nucleases are stimulated to cleave double-stranded (ds) DNA targets by a T-rich PAM, which restricts their targeting range. Here, we identify and characterize a new family of type V RNA-guided nuclease, Cas12l, that exclusively rec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ad27a91f59b7039c4602433f8e2cbba
https://pubmed.ncbi.nlm.nih.gov/36268581
https://pubmed.ncbi.nlm.nih.gov/36268581
Akademický článek
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Autor:
Priya, Vishnu, Singh, Sanjeev K., Revand, Ravindran, Kumar, Sandip, Mehata, Abhishesh Kumar, Sushmitha, Paulraj, Mahto, Sanjeev Kumar, Muthu, Madaswamy S.
Publikováno v:
Molecular Pharmaceutics; 1/2/2023, Vol. 20 Issue 1, p663-679, 17p
Autor:
Joshua K. Young, William E. Jack, Česlovas Venclovas, Clara M. Alarcon, Darius Kazlauskas, Zhenglin Hou, Virginijus Siksnys, Megumu Mabuchi, Tomas Urbaitis, Tautvydas Karvelis, G. Brett Robb, Mark Cigan, Shane K. Dooley, Monika Jasnauskaite, Giedrius Gasiunas, Ryan T. Fuchs, N. Doane Chilcoat, Greta Bigelyte, Mantvyda M. Grusyte, Ezra Schildkraut, Po-Hao Wang, Peter Weigele, Sushmitha Paulraj, Zhiyi Sun, Jennifer L. Curcuru
CRISPR-Cas9 nucleases are abundant in microbes. To explore this largely uncharacterized diversity, we applied cell-free biochemical screens to rapidly assess the protospacer adjacent motif (PAM) and guide RNA (gRNA) requirements of novel Cas9 protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f6cdbeb4449b33160674b8f8cb468bb
https://doi.org/10.1101/2020.04.29.066654
https://doi.org/10.1101/2020.04.29.066654
Autor:
Zhenglin Hou, Rimante Zedaveinyte, Joshua K. Young, Sushmitha Paulraj, Arunas Silanskas, Greta Bigelyte, Virginijus Siksnys, Vesna Djukanovic, Karolina Budre, Tautvydas Karvelis, Česlovas Venclovas, Stephen L. Gasior
Publikováno v:
Nucleic acids research, Oxford : Oxford University Press, 2020, first on line, p. [1-8]
Nucleic acids research, Oxford : Oxford University Press, 2020, vol. 48, iss. 9, p. 5016-5023
Nucleic Acids Research
Nucleic acids research, Oxford : Oxford University Press, 2020, vol. 48, iss. 9, p. 5016-5023
Nucleic Acids Research
In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d247b0d5509d3ee33c07486670fba61
https://repository.vu.lt/VU:ELABAPDB55056678&prefLang=en_US
https://repository.vu.lt/VU:ELABAPDB55056678&prefLang=en_US
Autor:
Zhenglin Hou, Česlovas Venclovas, Peter Weigele, Megumu Mabuchi, Joshua K. Young, William E. Jack, Zhiyi Sun, Greta Bigelyte, Tautvydas Karvelis, Ezra Schildkraut, Virginijus Siksnys, N. Doane Chilcoat, Tomas Urbaitis, G. Brett Robb, Ryan T. Fuchs, Monika Jasnauskaite, Jennifer L. Curcuru, Mantvyda M. Grusyte, Shane K. Dooley, Po-Hao Wang, Giedrius Gasiunas, Clara M. Alarcon, Darius Kazlauskas, Mark Cigan, Sushmitha Paulraj
Publikováno v:
Nature communications, London : Nature Publishing Group, 2020, vol. 11, no. 1, art. no. 5512, p. [1-10]
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been repurposed as genome editing tools. Although these proteins are found in many microbes, only a handful of variants are used for these applications. Here, we use bioi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c339dbfa2e08fc6e4b32aa0ea45d7144
https://repository.vu.lt/VU:ELABAPDB74530963&prefLang=en_US
https://repository.vu.lt/VU:ELABAPDB74530963&prefLang=en_US
Autor:
Stephen L. Gasior, Zhenglin Hou, Virginijus Siksnys, Greta Bigelyte, Vesna Djukanovic, Joshua K. Young, Arunas Silanskas, Česlovas Venclovas, Rimante Zedaveinyte, Sushmitha Paulraj, Karolina Budre, Tautvydas Karvelis
In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ae7b83f3950dd003dc996178de31ff0
https://doi.org/10.1101/654897
https://doi.org/10.1101/654897
Autor:
Brooke Peterson-Burch, N. Doane Chilcoat, Sandeep Kumar, Lijuan Wang, Stéphane Deschamps, Joshua Young, Sergei Svitashev, Clara M. Alarcon, Brian Lenderts, Sushmitha Paulraj, Virginijus Siksnys, Gina Zastrow-Hayes, Mindaugas Zaremba, Lanie Feigenbutz, Vesna Djukanovic, Gregory D. May, Ananta Acharya, Chris Schwartz
Publikováno v:
Scientific reports, London : Nature Publishing Group, 2019, vol. 9, art. no. 6729, p. [1-11]
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture productivity, but the possibility of unintended off-target edits has evoked some concerns. Here we employ a three-step strategy to investigate Cas9 nuclease specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::390840a4ab33ff12977b572e50054776
https://repository.vu.lt/VU:ELABAPDB42392648&prefLang=en_US
https://repository.vu.lt/VU:ELABAPDB42392648&prefLang=en_US