Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Sushil K. Thukral"'
Autor:
Edwin Rhim, Cheng Cheng, Sylvie Camier, Kenneth M. Dombek, Nataly Kacherovsky, Sushil K. Thukral, Elton T. Young
Publikováno v:
Molecular and Cellular Biology. 14:3842-3852
Adr1p is a regulatory protein in the yeast Saccharomyces cerevisiae that binds to and activates transcription from two sites in a perfect 22-bp inverted repeat, UAS1, in the ADH2 promoter. Binding requires two C2H2 zinc fingers and a region amino ter
Publikováno v:
Methods. 5:86-95
Molecular mechanisms of numerous cellular processes in eukaryotes are conserved between yeast and mammalian cells. Because of the ease of microbiological manipulation and genetic characterization, Saccharomyces cerevisiae is commonly used as a surrog
Mutations in the Zinc Fingers of ADR1 That Change the Specificity of DNA Binding and Transactivation
Publikováno v:
Molecular and Cellular Biology. 12:2784-2792
ADR1 is a yeast transcription factor that contains two zinc fingers of the Cys-2-His-2 (C2H2) class. Mutations that change the specificity of DNA binding of ADR1 to its target site, upstream activation sequence 1 (UAS1), have been identified at three
Publikováno v:
Molecular and Cellular Biology. 11:1566-1577
Publikováno v:
Molecular and Cellular Biology. 11:1566-1577
ADR1 is a transcription factor from Saccharomyces cerevisiae that regulates ADH2 expression through a 22-bp palindromic sequence (UAS1). Size fractionation studies revealed that full-length ADR1 and a truncated ADR1 protein containing the first 229 a
Autor:
JoAnn Tuan, Annick Itie, Michael Debbas, Athena W. Lin, Sushil K. Thukral, Andrew Wakeham, Tak W. Mak, Samuel Benchimol, Ying Lu, Scott W. Lowe, Seiji Kondo, Gary Elliott, Weili Ma, Ildiko Sarosi, Chris Saris
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 100(9)
The tumor suppressor p53 is regulated in part by binding to cellular proteins. We used p53 as bait in the yeast two-hybrid system and isolated homeodomain-interacting protein kinase 1 (HIPK1) as a p53-binding protein. Deletion analysis showed that am
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 88(20)
To identify functionally important amino acids in the two zinc fingers of transcription factor ADR1 [alcohol dehydrogenase (ADH) II synthesis regulator], oligonucleotide-directed mutagenesis was used to substitute alanine for the original amino acid
Publikováno v:
Biochemistry. 26:7883-7892
Publikováno v:
Molecular and Cellular Biology. 9:2360-2369
ADR1 is a transcription factor required for activation of the glucose-repressible alcohol dehydrogenase 2 (ADH2) gene in Saccharomyces cerevisiae. ADR1 has two zinc finger domains between amino acids 102 and 159, and it binds to an upstream activatio