Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Sushama Sivakumar"'
Autor:
Daniel A Armendariz, Sean C Goetsch, Anjana Sundarrajan, Sushama Sivakumar, Yihan Wang, Shiqi Xie, Nikhil V Munshi, Gary C Hon
Publikováno v:
eLife, Vol 12 (2023)
Enhancers orchestrate gene expression programs that drive multicellular development and lineage commitment. Thus, genetic variants at enhancers are thought to contribute to developmental diseases by altering cell fate commitment. However, while many
Externí odkaz:
https://doaj.org/article/b7f3ad57401f4ac983ed9e81a0c95e1e
Publikováno v:
STAR Protocols, Vol 3, Iss 4, Pp 101682- (2022)
Summary: Human embryonic stem cells (hESCs) continuously self-renew in culture and can be induced to differentiate into multiple cell types, including neural progenitor cells (NPCs). Here, we present a protocol to perform a CRISPR-Cas9 screen in hESC
Externí odkaz:
https://doaj.org/article/7b7bd851cf3b4b62ab1556187f652b8d
Autor:
Sushama Sivakumar, Gary J. Gorbsky
Publikováno v:
Biology Open, Vol 6, Iss 11, Pp 1672-1679 (2017)
Kinetochores move chromosomes on dynamic spindle microtubules and regulate signaling of the spindle checkpoint. The spindle- and kinetochore-associated (Ska) complex, a hexamer composed of two copies of Ska1, Ska2 and Ska3, has been implicated in bot
Externí odkaz:
https://doaj.org/article/9cd790ac385b40469d3c7885c50f558e
Autor:
Florian Kopp, Mahmoud M Elguindy, Mehmet E Yalvac, He Zhang, Beibei Chen, Frank A Gillett, Sungyul Lee, Sushama Sivakumar, Hongtao Yu, Yang Xie, Prashant Mishra, Zarife Sahenk, Joshua T Mendell
Publikováno v:
eLife, Vol 8 (2019)
Although numerous long noncoding RNAs (lncRNAs) have been identified, our understanding of their roles in mammalian physiology remains limited. Here, we investigated the physiologic function of the conserved lncRNA Norad in vivo. Deletion of Norad in
Externí odkaz:
https://doaj.org/article/11ac600402c64f12aeffc40cfa19a3d5
Autor:
Sushama Sivakumar, Paweł Ł Janczyk, Qianhui Qu, Chad A Brautigam, P Todd Stukenberg, Hongtao Yu, Gary J Gorbsky
Publikováno v:
eLife, Vol 5 (2016)
The spindle- and kinetochore-associated (Ska) complex is essential for normal anaphase onset in mitosis. The C-terminal domain (CTD) of Ska1 binds microtubules and was proposed to facilitate kinetochore movement on depolymerizing spindle microtubules
Externí odkaz:
https://doaj.org/article/b9e3beb34c044d49830a9befa8858711
Autor:
Sean C Goetsch, Daniel A Armendariz, Anjana Sundarrajan, Sushama Sivakumar, Yihan Wang, Shiqi Xie, Nikhil V Munshi, Gary C Hon
Publikováno v:
eLife. 12
Enhancers orchestrate gene expression programs that drive multicellular development and lineage commitment. Thus, genetic variants at enhancers are thought to contribute to developmental diseases by altering cell fate commitment. However, while many
Publikováno v:
Methods Mol Biol
Methods in Molecular Biology ISBN: 9781071619032
Methods in Molecular Biology ISBN: 9781071619032
Live--cell fluorescence microscopy is an effective tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors and after RNA interference--mediated or CRISPR knockout--mediated depletion of protein targets. Live
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74b6426fa92c9d8cf56bc0050c9df660
https://europepmc.org/articles/PMC10092068/
https://europepmc.org/articles/PMC10092068/
Autor:
Hongtao Yu, Chao Xing, Sushama Sivakumar, Mohammed Kanchwala, Shutao Qi, Bret M. Evers, Ningyan Cheng, Adwait Amod Sathe, Ashwani Kumar
SUMMARYAneuploidy, defective differentiation, and inactivation of the tumor suppressor TP53 all occur frequently during tumorigenesis. Here, we probe the potential links among these cancer traits by inactivating TP53 in human embryonic stem cells (hE
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c162600bf142dc829a266e9f22831ce5
https://doi.org/10.1101/2021.07.22.453348
https://doi.org/10.1101/2021.07.22.453348
Autor:
Sushama Sivakumar, Shutao Qi, Ningyan Cheng, Adwait A. Sathe, Mohammed Kanchwala, Ashwani Kumar, Bret M. Evers, Chao Xing, Hongtao Yu
Publikováno v:
Cell reports. 38(7)
Aneuploidy, defective differentiation, and inactivation of the tumor suppressor TP53 all occur frequently during tumorigenesis. Here, we probe the potential links among these cancer traits by inactivating TP53 in human embryonic stem cells (hESCs). T