Zobrazeno 1 - 10
of 97
pro vyhledávání: '"Susanne Klumpp"'
Publikováno v:
PLoS ONE, Vol 6, Iss 8, p e23612 (2011)
Protein histidine phosphatase (PHP) is an enzyme which removes phosphate groups from histidine residues. It was described for vertebrates in the year 2002. The recombinant human 16 kDa protein forms multimeric complexes in physiological buffer and in
Externí odkaz:
https://doaj.org/article/b573ed1407a74b66bcd7619e0f6994f2
Autor:
Josef Krieglstein, Tobias Kewitz, Uwe Kirchhefer, Oliver Hofnagel, Gabriele Weissen-Plenz, Michael Reinbold, Susanne Klumpp
Publikováno v:
PLoS ONE, Vol 5, Iss 3, p e9561 (2010)
BackgroundMono-unsaturated fatty acids (MUFAs) like oleic acid have been shown to cause apoptosis of cultured endothelial cells by activating protein phosphatase type 2C alpha and beta (PP2C). The question arises whether damage of endothelial or othe
Externí odkaz:
https://doaj.org/article/640c217328584e2bbf7d3c91fcdc2398
Publikováno v:
Cell Biol. Int. 36, 245-249 (2012)
Protein histidine phosphatase (PHP) discovered previously has been shown to be expressed in mammalian tissues. Particularly, in blood vessel walls PHP was markedly expressed. Therefore, we made an attempt to find out whether PHP plays a significant r
Autor:
Bhavaani Jayaram, Josef Krieglstein, Katrin Ludwig, Anjaneyulu Kowluru, Ismail Syed, Vasudeva Kamath, Susanne Klumpp, Chandrashekara Kyathanahalli, Lawrence Karl Olson
Publikováno v:
American Journal of Physiology-Endocrinology and Metabolism. 299:E276-E286
We report localization of a cytosolic protein histidine phosphatase (PHP; approximately 16 kDa) in INS 832/13 cells, normal rat islets, and human islets. siRNA-mediated knockdown of PHP markedly reduced glucose- or mitochondrial fuel-induced but not
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1804:206-211
There is increasing evidence that reversible phosphorylation of histidine residues regulates numerous important cellular processes. The first protein histidine phosphatase (PHP) from vertebrates was discovered just recently. Here, we report on amino
Autor:
Paul V. Attwood, Klaus Bergander, Josef Krieglstein, Katrin Ludwig, Paul G. Besant, Susanne Klumpp, Abdussalam Adina-Zada
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1804:199-205
Using peptides based on the amino acid sequences surrounding the two histidine residues in histone H4, we have investigated the kinetics of the phosphorylation and dephosphorylation reactions of their histidine residues, when reacted with potassium p
Publikováno v:
Neuroscience. 164:1695-1700
The neurotrophic and neuroprotective activity of fibroblast growth factor (FGF2) is well documented. In this study, we attempted to demonstrate that binding of ATP to FGF2 is essential for its neuroprotective effect. Incubation of primary cultures of
Publikováno v:
Brain Research. 1264:7-12
We recently found protein histidine phosphatase (PHP) in eukaryotes and identified ATP- citrate lyase (ACL) and the β-subunit of G-proteins as its substrates. The aim of the present study was to get information on the significance of PHP for cellula
Autor:
Heiko Hayen, Susanne Klumpp, Josef Krieglstein, Eva-Maria Hein, Daniel Fischer, Nils Helge Schebb, Uwe Karst
Publikováno v:
Journal of Chromatography A. 1183:100-107
A fast liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed for the analysis of acetylcholine (ACh) in cultured cells. [ 2 H 4 ]Acetylcholine (ACh- d 4 ) was used as an internal standard for calibration. ACh was extracte
Publikováno v:
Journal of the American Society for Mass Spectrometry. 19:91-95
It was shown in previous work that the interaction of growth factors (GFs) with adenosine triphosphate (ATP) is essential for their neuroprotective effect. Here we investigated the nature of the association of human basic fibroblast growth factor (bF