Recent Developments and Strategies for the Application of Agrobacterium-Mediated Transformation of Apple Malus × domestica Borkh

Autor: Susan Schröpfer, Janne Lempe, Ofere Francis Emeriewen, Henryk Flachowsky

Publikováno v: Frontiers in Plant Science, Vol 13 (2022)

Genetic transformation has become an important tool in plant genome research over the last three decades. This applies not only to model plants such as Arabidopsis thaliana but also increasingly to cultivated plants, where the establishment of transf

Externí odkaz: https://doaj.org/article/490e407de4264968852dbd0d23fc8e50

A Single Effector Protein, AvrRpt2EA, from Erwinia amylovora Can Cause Fire Blight Disease Symptoms and Induces a Salicylic Acid–Dependent Defense Response

Autor: Susan Schröpfer, Christoph Böttcher, Thomas Wöhner, Klaus Richter, John Norelli, Erik H. A. Rikkerink, Magda-Viola Hanke, Henryk Flachowsky

Publikováno v: Molecular Plant-Microbe Interactions, Vol 31, Iss 11, Pp 1179-1191 (2018)

The AvrRpt2EA effector protein of Erwinia amylovora is important for pathogen recognition in the fire blight–resistant crabapple Malus × robusta 5; however, little is known about its role in susceptible apples. To study its function in planta, we

Externí odkaz: https://doaj.org/article/0f6be199a0884f96900dabbb30588d2e

The topoisomerase 3α zinc-finger domain T1 of Arabidopsis thaliana is required for targeting the enzyme activity to Holliday junction-like DNA repair intermediates.

Autor: Annika Dorn, Sarah Röhrig, Kristin Papp, Susan Schröpfer, Frank Hartung, Alexander Knoll, Holger Puchta

Publikováno v: PLoS Genetics, Vol 14, Iss 9, p e1007674 (2018)

Topoisomerase 3α, a class I topoisomerase, consists of a TOPRIM domain, an active centre and a variable number of zinc-finger domains (ZFDs) at the C-terminus, in multicellular organisms. Whereas the functions of the TOPRIM domain and the active cen

Externí odkaz: https://doaj.org/article/0099a6a14c644ed1a7fe2062f6e88c54

Tracing CRISPR/Cas12a Mediated Genome Editing Events in Apple Using High-Throughput Genotyping by PCR Capillary Gel Electrophoresis

Autor: Susan Schröpfer, Henryk Flachowsky

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 12611, p 12611 (2021)
International Journal of Molecular Sciences; Volume 22; Issue 22; Pages: 12611
International Journal of Molecular Sciences

The use of the novel CRISPR/Cas12a system is advantageous, as it expands the possibilities for genome editing (GE) applications due to its different features compared to the commonly used CRISPR/Cas9 system. In this work, the CRISPR/Cas12a system was

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c78b8a9e3c59f00608af0319d03ca45
https://www.mdpi.com/1422-0067/22/22/12611

Transcriptional profile of AvrRpt2EA-mediated resistance and susceptibility response to Erwinia amylovora in apple

Autor: Henryk Flachowsky, Susan Schröpfer, Isabelle Vogt, Andreas Peil, Klaus Richter, Giovanni A. L. Broggini, Andreas Dahl, Magda-Viola Hanke

Publikováno v: Scientific Reports, 11 (1)
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

Most of the commercial apple cultivars are highly susceptible to fire blight, which is the most devastating bacterial disease affecting pome fruits. Resistance to fire blight is described especially in wild Malus accessions such as M. × robusta 5 (M

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f68bdd7eb286c5cf910fec4f5dc44a0d
https://hdl.handle.net/20.500.11850/482495