Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Susan N. Meloan"'
Publikováno v:
Journal of Histotechnology. 12:129-134
Differences among the useful lifetimes of stains are well known to histotechnologists. Though problems of fading have been studied extensively by chemists, these data received little attention in histotechnology. Yet, such information is invaluable f
Autor:
Susan N. Meloan, Holde Puchtler
Publikováno v:
Journal of Histotechnology. 11:153-157
In his phosphotungstic acid-hematein (PTAH) formula, Mallory combined hematoxylin with phosphotungstic acid (PTA) and then added the oxidant potassium permanganate. As hematoxylin is oxidized, protons of the strongly acid PTA compete with the metal f
Publikováno v:
Histochemistry. 45:255-265
A variety of structural formulas has been suggested for the basic fuchsin moiety of aldehyde-Schiff reaction products. It was therefore deemed of interest to review the development of these different concepts of dye structure. Formulation of basic fu
Publikováno v:
Journal of Histotechnology. 12:57-61
Some dyes used in histotechnology fade within months; others remain unaltered for many years. Problems of fading are well known since antiquity and have been thoroughly investigated. Yet textbooks of histotechnology do not provide information concern
Publikováno v:
Histochemistry. 44:105-118
Cytoplasmic filaments have been studied extensively by electron microscopy, but the histochemical nature of such fibrils in non-keratinizing epithelia has not been systematically investigated. During studies of early arterial lesions we observed stru
Autor:
Susan N. Meloan, Holde Puchtler
Publikováno v:
Stain Technology. 49:301-304
Absrract. Recent shortages of hematoxylin have made it desirable to find substitutes for staining of nuclei. Iron alizarin blue S proved practical. Formula: Add 5 mi of a 40% femc chloride solution to 100 ml of a 2% aqueous solution of alizarin blue
Publikováno v:
Histochemistry. 88:243-256
Previous studies of picro-dye reactions demonstrated wide variations in the binding of different dyes. Picro-Sirius Red F3BA was recommended because it colors all collagens intensely and is suitable for polarization microscopy. Recent publications on
Publikováno v:
Journal of Histotechnology. 7:123-126
Conventional Thioflavine T and S stains for fluorescence microscopy of amyloid require differentiation and are not specific. The selective fluorescent brightening agent Phorwhite BBU is currently unavailable on the biological dye market. We therefore
Publikováno v:
Histochemistry. 49:1-14
Distinction between elastin and collagen in arteriosclerotic lesions is difficult because the so-called elastica stains are bound also by collagen fibers which resemble collagen of premature infants. Investigations of effects of organic solvents on d
Publikováno v:
Journal of Histotechnology. 7:181-185
Gomori's one-step trichrome stain eliminated vagaries of differentiation inherent in multi-step methods, but fine connective tissue fibers are sometimes poorly stained. Lowering the pH of the dye solution to 1.3 yielded uniform coloration of Carnoy-f