Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Susan E. Sefers"'
Autor:
Susan E. Sefers, Jonathan E. Schmitz
Publikováno v:
Advanced Techniques in Diagnostic Microbiology ISBN: 9783319338996
Laboratory strategies for diagnosing and monitoring infections now routinely include nucleic acid amplification techniques (NAATs), in particular variations of the polymerase chain reaction. These molecular approaches represent a paradigm shift and c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::57f6562bfdc032dac55e7d061739cb38
https://doi.org/10.1007/978-3-319-33900-9_24
https://doi.org/10.1007/978-3-319-33900-9_24
Autor:
Yi-Wei Tang, Robert Lovari, Susan E. Sefers, Criziel D. Quinn, Shufang Meng, Haijing Li, Jeong Hwan Shin, Raymond Ranken, Heather E. Carolan, Christian Massire, Charles W. Stratton, Donna Toleno, Jeong Nyeo Lee
Publikováno v:
Journal of Clinical Microbiology. 51:136-141
As pulmonary fungal infections continue to increase due to an increasing number of immunocompromised patients, rapid detection and accurate identification of these fungal pathogens are critical. A broad fungal assay was developed by incorporating bro
Autor:
James D. Chappell, Melinda A. McCormac, Haijing Li, R. Wray Estes, Susan E. Sefers, Yi-Wei Tang, Peter F. Wright, Dean D. Erdman, Ryan K Dare
Publikováno v:
Journal of Clinical Microbiology
Clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically desirable. We evaluated two multiplex reverse trans
Autor:
Yi-Wei Tang, Elizabeth R. Unger, Jian Han, Sharon J. Smith, David C. Swan, Shanjuan H. Lum, Susan E. Sefers
Publikováno v:
Journal of Clinical Microbiology. 44:4157-4162
The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tu
Autor:
Jamie Rickmyre, Haijing Li, Susan E. Sefers, Kathryn M. Edwards, Amondrea Blackman, Yi-Wei Tang
Publikováno v:
Journal of Clinical Virology
Background Nucleic acid preparation from a variety of clinical specimens requires efficient target recovery and amplification inhibitor removal and is critical for successful molecular diagnosis. The QIAamp MinElute Virus kit (Qiagen Inc., Valencia,
Publikováno v:
Journal of Clinical Microbiology. 43:4830-4833
A nucleic acid extraction system that can handle small numbers of specimens with a short test turnaround time and short hands-on time is desirable for emergent testing. We performed a comparative validation on three systems: the MagNA Pure compact sy
Publikováno v:
Reviews in Medical Microbiology. 16:59-67
Nucleic acid-based tests are rapidly expanding in the field of diagnostic microbiology, due to their unique high sensitivity and specificity as well as rapid assay turnaround time. However, the potential of false positives and false negatives can hin
Publikováno v:
Advanced Techniques in Diagnostic Microbiology ISBN: 9781461439691
“With great power comes great responsibility.” This popular Spider Man movie quote infers the relationship between molecular nucleic acid amplification methods and amplification product inactivation procedures. The “power” is the ability of m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::58428bd82d21a30cfacad631aad97132
https://doi.org/10.1007/978-1-4614-3970-7_26
https://doi.org/10.1007/978-1-4614-3970-7_26
Publikováno v:
Diagnostic Virology Protocols ISBN: 9781607618164
Rapid and accurate detection of respiratory syncytial virus (RSV) provides pathogen-specific diagnosis, allows implementation of appropriate infection control measures, and improves patient management. One diagnostic challenge is that respiratory inf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01a737b75a18b9b4a26664ed3cb2ad69
https://doi.org/10.1007/978-1-60761-817-1_17
https://doi.org/10.1007/978-1-60761-817-1_17
Autor:
Susan E. Sefers, Shufang Meng, Heather E. Matthews, Chris D. Crowder, Charles W. Stratton, Lawrence B. Blyn, Yi-Wei Tang, Haijing Li, Mark W. Eshoo, Rangarajan Sampath, David J. Ecker
Publikováno v:
Journal of clinical microbiology. 48(2)
Rapid detection and identification of Ehrlichia species improves clinical outcome for patients suspected of ehrlichiosis. We describe an assay that employs multilocus PCR and electrospray ionization mass spectrometry (PCR/ESI-MS) to detect and identi