Unlocking the potential of large-scale 3D imaging with tissue clearing techniques.

Autor: Susaki EA; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, 2-2-1, Hongo, Bunkyo-ku, Tokyo, Japan.; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, 2-2-1, Hongo, Bunkyo-ku, Tokyo, Japan.; RIKEN Center for Biosystems Dynamics Research, 1-3 Yamadaoka, Suita, Osaka, Japan.; Institute for Quantum Life Science, National Institutes for Quantum Science and Technology, 4-9-1, Anagawa, Inage-ku, Chiba, Japan.

Publikováno v: Microscopy (Oxford, England) [Microscopy (Oxf)] 2024 Sep 28. Date of Electronic Publication: 2024 Sep 28.

Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation.

Autor: Kon K; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Kennedy Krieger Institute, Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Ode KL; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan., Mano T; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; Department of Information Physics and Computing, Graduate School of Information Science and Technology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Computational Neuroethology Unit, Okinawa Institute of Science and Technology, Onna, Okinawa, Japan., Fujishima H; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; Department of Systems Biology, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan., Takahashi RR; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Tone D; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan., Shimizu C; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan., Shiono S; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Yada S; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; International Institute for Integrative Sleep Medicine (IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan., Matsuzawa K; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan., Yoshida SY; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan., Yoshida Garçon J; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; Department of Molecular Embryology, Research Institute, Osaka Women's and Children's Hospital, Izumi, Osaka, Japan., Kaneko M; Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Biosystems Dynamics Research (BDR), Chuou-ku, Kobe, Hyogo, Japan., Shinohara Y; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; Division of Molecular Psychoimmunology, Institute for Genetic Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan., Yamada RG; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; Department of Systems Biology, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan., Shi S; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; International Institute for Integrative Sleep Medicine (IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan., Miyamichi K; Laboratory for Comparative Connectomics, RIKEN Center for Biosystems Dynamics Research (BDR), Chuou-ku, Kobe, Hyogo, Japan., Sumiyama K; Laboratory for Mouse Genetic Engineering, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; Laboratory of Animal Genetics and Breeding, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan., Kiyonari H; Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Biosystems Dynamics Research (BDR), Chuou-ku, Kobe, Hyogo, Japan., Susaki EA; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan.; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan., Ueda HR; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. uedah-tky@umin.ac.jp.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka, Japan. uedah-tky@umin.ac.jp.; Department of Information Physics and Computing, Graduate School of Information Science and Technology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. uedah-tky@umin.ac.jp.; Department of Systems Biology, Institute of Life Science, Kurume University, Kurume, Fukuoka, Japan. uedah-tky@umin.ac.jp.

Publikováno v: Nature communications [Nat Commun] 2024 Jul 18; Vol. 15 (1), pp. 6054. Date of Electronic Publication: 2024 Jul 18.

descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.

Autor: Otomo K; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Biochemistry II, Juntendo University School of Medicine, Tokyo, Japan.; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.; Biophotonics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan., Omura T; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Department of Neurosurgery, University of Tokyo, Tokyo, Japan.; Department of Neurosurgery and Neuro-Oncology, National Cancer Center Hospital, Tokyo, Japan., Nozawa Y; Biochemistry II, Juntendo University School of Medicine, Tokyo, Japan., Edwards SJ; Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology, Stockholm, Sweden., Sato Y; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan., Saito Y; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan., Yagishita S; Department of Pharmacology and Therapeutics, Fundamental Innovative Oncology Core, National Cancer Center Research Institute, Tokyo, Japan.; Division of Molecular Pharmacology, National Cancer Center Research Institute, Tokyo, Japan., Uchida H; Department of System Pathology for Neurological Disorders, Brain Research Institute, Niigata University, Niigata, Japan., Watakabe Y; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.; Biophotonics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan., Naitou K; Department of Basic Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan., Yanai R; Advanced Neuroimaging Center, Institute for Quantum Medical Sciences, National Institutes for Quantum Science and Technology, Chiba, Japan., Sahara N; Advanced Neuroimaging Center, Institute for Quantum Medical Sciences, National Institutes for Quantum Science and Technology, Chiba, Japan., Takagi S; Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan., Katayama R; Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan., Iwata Y; Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan., Shiokawa T; Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan., Hayakawa Y; Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan., Otsuka K; Biology and Environmental Chemistry Division, Sustainable System Research Laboratory, Central Research Institute of Electric Power Industry, Chiba, Japan., Watanabe-Takano H; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, Tokyo, Japan., Haneda Y; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, Tokyo, Japan., Fukuhara S; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, Tokyo, Japan., Fujiwara M; Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan., Nii T; Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan., Meno C; Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan., Takeshita N; Anatomy and Developmental Biology, Kyoto Prefectural University of Medicine, Kyoto, Japan.; Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto, Japan., Yashiro K; Anatomy and Developmental Biology, Kyoto Prefectural University of Medicine, Kyoto, Japan., Rosales Rocabado JM; Division of Bio-prosthodontics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan., Kaku M; Division of Bio-prosthodontics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan., Yamada T; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, USA., Oishi Y; Department of Meidical Biochemistry, Tokyo Medical and Dental University, Tokyo, Japan., Koike H; Department of Meidical Biochemistry, Tokyo Medical and Dental University, Tokyo, Japan., Cheng Y; Department of Meidical Biochemistry, Tokyo Medical and Dental University, Tokyo, Japan., Sekine K; Laboratory of Cancer Cell Systems, National Cancer Center Research Institute, Tokyo, Japan., Koga JI; The Second Department of Internal Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan., Sugiyama K; Institute for Advanced Research of Biosystem Dynamics, Research Institute for Science and Engineering, Waseda University, Tokyo, Japan., Kimura K; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan., Karube F; Lab of Histology and Cytology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan., Kim H; Department of Systems Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan., Manabe I; Department of Systems Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan., Nemoto T; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.; Biophotonics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan., Tainaka K; Department of System Pathology for Neurological Disorders, Brain Research Institute, Niigata University, Niigata, Japan., Hamada A; Department of Pharmacology and Therapeutics, Fundamental Innovative Oncology Core, National Cancer Center Research Institute, Tokyo, Japan.; Division of Molecular Pharmacology, National Cancer Center Research Institute, Tokyo, Japan., Brismar H; Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology, Stockholm, Sweden., Susaki EA; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo, Japan. suishess-kyu@umin.ac.jp.; Biochemistry II, Juntendo University School of Medicine, Tokyo, Japan. suishess-kyu@umin.ac.jp.; Nakatani Biomedical Spatialomics Hub, Juntendo University Graduate School of Medicine, Tokyo, Japan. suishess-kyu@umin.ac.jp.

Publikováno v: Nature communications [Nat Commun] 2024 Jun 12; Vol. 15 (1), pp. 4941. Date of Electronic Publication: 2024 Jun 12.

Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains.

Autor: Nonaka H; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.; Hamachi Innovative Molecular Technology for Neuroscience, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kyoto 615-8530, Japan., Sakamoto S; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.; Hamachi Innovative Molecular Technology for Neuroscience, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kyoto 615-8530, Japan., Shiraiwa K; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan., Ishikawa M; Hamachi Innovative Molecular Technology for Neuroscience, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kyoto 615-8530, Japan., Tamura T; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.; Hamachi Innovative Molecular Technology for Neuroscience, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kyoto 615-8530, Japan., Okuno K; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan., Kondo T; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan., Kiyonaka S; Hamachi Innovative Molecular Technology for Neuroscience, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kyoto 615-8530, Japan.; Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan., Susaki EA; Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan.; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka 565-5241, Japan., Shimizu C; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka 565-5241, Japan., Ueda HR; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka 565-5241, Japan.; Department of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan., Kakegawa W; Hamachi Innovative Molecular Technology for Neuroscience, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kyoto 615-8530, Japan.; Department of Neurophysiology, Keio University School of Medicine, Tokyo 160-8582, Japan., Arai I; Department of Neurophysiology, Keio University School of Medicine, Tokyo 160-8582, Japan., Yuzaki M; Department of Neurophysiology, Keio University School of Medicine, Tokyo 160-8582, Japan., Hamachi I; Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.; Hamachi Innovative Molecular Technology for Neuroscience, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Kyoto 615-8530, Japan.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Feb 06; Vol. 121 (6), pp. e2313887121. Date of Electronic Publication: 2024 Jan 31.

Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging.

Autor: Prince MNH; Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA., Garcia B; Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA., Henn C; Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA., Yi Y; Chinese Institute for Brain Research, Beijing 102206, China., Susaki EA; Department of Biochemistry and Systems Biomedicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan., Watakabe Y; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 5-1 Higashiyama, Okazaki, Aichi, 444-8787, Japan.; Biophotonics Research Group, Exploratory Research Center for Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Okazaki, Aichi, 444-8787, Japan., Nemoto T; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 5-1 Higashiyama, Okazaki, Aichi, 444-8787, Japan.; Biophotonics Research Group, Exploratory Research Center for Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Okazaki, Aichi, 444-8787, Japan., Lidke KA; Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA., Zhao H; Chinese Institute for Brain Research, Beijing 102206, China., Remiro IS; Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA., Liu S; Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA., Chakraborty T; Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA.; Comprehensive Cancer Center, University of New Mexico, Albuquerque, NM 87102, USA.

Publikováno v: Research square [Res Sq] 2023 Jun 28. Date of Electronic Publication: 2023 Jun 28.