Heterologous expression of the antimyotoxic protein DM64 in Pichia pastoris.

Autor: Saulo Martins Vieira, Surza Lucia Gonçalves da Rocha, Ana Gisele da Costa Neves-Ferreira, Rodrigo Volcan Almeida, Jonas Perales

Publikováno v: PLoS Neglected Tropical Diseases, Vol 11, Iss 7, p e0005829 (2017)

Snakebite envenomation is a neglected condition that constitutes a public health problem in tropical and subtropical countries, including Brazil. Interestingly, some animals are resistant to snake envenomation due to the presence of inhibitory glycop

Externí odkaz: https://doaj.org/article/42446813a286465f9390a1c8f8b51cfd

ASP-Enzymosomes with Saccharomyces cerevisiae Asparaginase II Expressed in Pichia pastoris: Formulation Design and In Vitro Studies of a Potential Antileukemic Drug

Autor: M. Bárbara F. Martins, Elba P. S. Bon, M. Luísa Corvo, Surza Lucia Gonçalves da Rocha, Manuela Carvalheiro, Luciana Facchinetti de Castro Girão, Maria Antonieta Ferrara, Margarida Ferreira-Silva, Jonas Perales

Publikováno v: International Journal of Molecular Sciences, Vol 22, Iss 11120, p 11120 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 20

The bacterial enzyme asparaginase is the main treatment option for acute lymphoblastic leukemia. However, it causes side effects, such as immunological reactions, and presents undesirable glutaminase activity. As an alternative, we have been studying

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ab36a4e28bb06f44771f7cd0263bbe2
https://www.mdpi.com/1422-0067/22/20/11120

Screening for target toxins of the antiophidic protein DM64 through a gel-based interactomics approach

Autor: Jonas Perales, Yamileth Angulo, Surza Lucia Gonçalves da Rocha, Bruno Lomonte, Gilberto B. Domont, Monique R.O. Trugilho, Ana G.C. Neves-Ferreira, Richard H. Valente

Publikováno v: Journal of Proteomics, Vol 151, pp 204-213
Kérwá
Universidad de Costa Rica
instacron:UCR

DM64 is a glycosylated protein with antivenomactivity isolated fromthe serum of the opossumDidelphis aurita. It binds non-covalently to myotoxins I (Asp49) and II (Lys49) from Bothrops asper venom and inhibits their myotoxic effect. In this study, an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c12df05e74a36a8ed4a8feef6936779a
https://doi.org/10.1016/j.jprot.2016.05.020

Structural analysis of a myotoxin-antimyotoxin complex by cross-linking, mass spectrometry, and bioinformatics

Autor: Barbara Da Silva Soares, Jonas Perales, Surza Lucia Gonçalves da Rocha, Ana Gisele Da Costa Neves Ferreira, Mariana Fioramonte, Francisco Gomes-Neto, Fabio C. Gozzo, Bruno Lomonte, Diogo B. Lima, Richard H. Valente

Publikováno v: Toxicon. 158:S29-S30

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::30a1b9921be33f7bfff11c913f6182fc
https://doi.org/10.1016/j.toxicon.2018.10.106

Modeling Protein-Protein Interactions: a structural insight of myotoxin-antimyotoxin complex based on cross-linking data, resolved by mass spectrometry

Autor: Barbara Da Silva Soares, Diogo B. Lima, Fabio C. Gozzo, Bruno Lomonte, Richard H. Valente, Jonas Perales, Surza Lucia Gonçalves da Rocha, Ana G.C. Neves-Ferreira, Francisco Gomes Neto

Publikováno v: Toxicon. 168:S27

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0e96fb346de51116911feb79557d5b30
https://doi.org/10.1016/j.toxicon.2019.06.116