An Automated Scientist to Design and Optimize Microbial Strains for the Industrial Production of Small Molecules

Autor: Amoolya H. Singh, Benjamin B. Kaufmann-Malaga, Joshua A. Lerman, Daniel P. Dougherty, Yang Zhang, Alexander L. Kilbo, Erin H. Wilson, Chiam Yu Ng, Onur Erbilgin, Kate A. Curran, Christopher D. Reeves, John E. Hung, Simone Mantovani, Zachary A. King, Marites J. Ayson, Judith R. Denery, Chia-Wei Lu, Phillip Norton, Carol Tran, Darren M. Platt, Joel R. Cherry, Sunil S. Chandran, Adam L. Meadows

Engineering microbes to synthesize molecules of societal value has historically been a time consuming and artisanal process, with the synthesis of each new non-native molecule typically warranting its own separate publication. Because most microbial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::494b4676c035c6da704381c0cce91660
https://doi.org/10.1101/2023.01.03.521657

Method for Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas9

Autor: Kristy Michelle Hawkins, Darren Platt, Max G. Schubert, Sunil S. Chandran, Andrew A. Horwitz, Jack D. Newman, Tina Mahatdejkul-Meadows, Jessica M. Walter, Stephanie H. Kung, Aaron D. Hernday, Wayne Szeto

Publikováno v: Methods in Molecular Biology ISBN: 9781493997350

CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ad30205bac9a685c020b058739f269cf
https://doi.org/10.1007/978-1-4939-9736-7_3

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

Autor: Aaron D. Hernday, Sunil S. Chandran, Stephanie H. Kung, Wayne Szeto, Jessica M. Walter, Max G. Schubert, Tina Mahatdejkul-Meadows, Kristy Michelle Hawkins, Darren Platt, Jack D. Newman, Andrew A. Horwitz

Publikováno v: Horwitz, AA; Walter, JM; Schubert, MG; Kung, SH; Hawkins, K; Platt, DM; et al.(2015). Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas. Cell Systems, 1(1), 88-96. doi: 10.1016/j.cels.2015.02.001. UC Merced: Retrieved from: http://www.escholarship.org/uc/item/01d6s8m1
Cell systems, vol 1, iss 1

© 2015 The Authors. Summary CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression plasmids for multiplexed gene knockouts and point mutations. Here we show that co-transformation of a single linearized plasmid with m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d27ed41b04db0f82fc173913678fa9e8
https://doi.org/10.1016/j.cels.2015.02.001

Rapid Assembly of DNA via Ligase Cycling Reaction (LCR)

Autor: Sunil S. Chandran

Publikováno v: Methods in Molecular Biology ISBN: 9781493963416

The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the ligase chain reaction (LCR). The LCR m

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1269093ff2b5b63caee0bec2e8ae4fbb
https://doi.org/10.1007/978-1-4939-6343-0_8

Combinatorial polyketide biosynthesis by de novo design and rearrangement of modular polyketide synthase genes

Autor: Kedar G. Patel, Ralph Reid, Sunil S. Chandran, Daniel V. Santi, Hugo G. Menzella, Sarah J. Reisinger, David A. Hopwood, John R. Carney

Publikováno v: Nature Biotechnology. 23:1171-1176

Type I polyketide synthase (PKS) genes consist of modules approximately 3-6 kb long, which encode the structures of 2-carbon units in polyketide products. Alteration or replacement of individual PKS modules can lead to the biosynthesis of 'unnatural'

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::667753fe15a2afd52c0463886e0abaea
https://doi.org/10.1038/nbt1128