Membrane anchoring facilitates colocalization of enzymes in plant cytochrome P450 redox systems

Autor: Tomas Laursen, Hiu Yue Monatrice Lam, Kasper Kildegaard Sørensen, Pengfei Tian, Cecilie Cetti Hansen, Jay T. Groves, Knud Jørgen Jensen, Sune M. Christensen

Publikováno v: Communications Biology, Vol 4, Iss 1, Pp 1-9 (2021)

Laursen et al. investigates the role of membrane anchoring for the organization and efficiency of P450-driven plant metabolism. They report an original experimental design to monitor interactions between anchoring segments of membrane-bound enzymes a

Externí odkaz: https://doaj.org/article/3e1e47113b9c433fa180873c4a5ccde7

Sensing-Applications of Surface-Based Single Vesicle Arrays

Autor: Sune M. Christensen, Dimitrios G. Stamou

Publikováno v: Sensors, Vol 10, Iss 12, Pp 11352-11368 (2010)

A single lipid vesicle can be regarded as an autonomous ultra-miniaturised 3D biomimetic “scaffold” (Ø ≥ 13 nm) ideally suited for reconstitution and interrogation of biochemical processes. The enclosing lipid bilayer membrane of a vesicle can

Externí odkaz: https://doaj.org/article/8071296836cc47b68d850919e71bf1af

Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition

Autor: Allan Svendsen, Johannes Thomsen, Søren S.-R. Bohr, Nikos S. Hatzakis, Henrik Dahl Pinholt, Sune M. Christensen, Amalie S. Kallenbach, Lars Iversen, Philip M. Lund

Publikováno v: Bohr, S S-R, Lund, P M, Kallenbach, A S, Pinholt, H, Thomsen, J, Iversen, L, Svendsen, A, Christensen, S M & Hatzakis, N S 2019, ' Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition ', Scientific Reports, vol. 9, 16169 . https://doi.org/10.1038/s41598-019-52539-1
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)

Lipases are interfacially activated enzymes that catalyze the hydrolysis of ester bonds and constitute prime candidates for industrial and biotechnological applications ranging from detergent industry, to chiral organic synthesis. As a result, there

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a92db2807417714a506d286593b8a73
https://doi.org/10.1038/s41598-019-52539-1

One-way membrane trafficking of SOS in receptor-triggered Ras activation

Autor: Steven Alvarez, Dafna Bar-Sagi, Jeffrey S. Iwig, Jay T. Groves, Jesse E. Jun, Hsiung-Lin Tu, Sune M. Christensen, Meredith G. Triplet, Kamlesh K Yadav, Jeroen P. Roose

Publikováno v: Nature structural & molecular biology

SOS is a key activator of the small GTPase Ras. In cells, SOS-Ras signaling is thought to be initiated predominantly by membrane recruitment of SOS via the adaptor Grb2 and balanced by rapidly reversible Grb2-SOS binding kinetics. However, SOS has mu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1abbb2f85f39ffe26453c8db9da1adfe
https://doi.org/10.1038/nsmb.3275

Direct observation of proton pumping by a eukaryotic P-type ATPase

Autor: Andreas L. Christensen, Bo Højen Justesen, Nikos S. Hatzakis, Sune M. Christensen, Gerdi Christine Kemmer, Christina Lohr, Michael Grabe, Ida Louise Jørgensen, Salome Veshaguri, Jürgen Schiller, Dimitrios Stamou, Garima Ghale, Thomas Günther Pomorski, Mads P. Møller

Publikováno v: Science. 351:1469-1473

In eukaryotes, P-type adenosine triphosphatases (ATPases) generate the plasma membrane potential and drive secondary transport systems; however, despite their importance, their regulation remains poorly understood. We monitored at the single-molecule

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05832a3f6c32edde053f19ee08df16e3
https://doi.org/10.1126/science.aad6429