Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Sun C. Kim"'
Publikováno v:
Journal of Molecular Biology. 258:638-649
The Fok I restriction endonuclease recognizes the double-stranded (ds) 5′-GGATG-3′ site and cuts at the 9th and 13th nucleotides downstream from the 5′-3′ and 3′-5′ strands, respectively. To elucidate the interaction between Fok I and DNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d87703284b89a7dd0306778896b1a78d
https://doi.org/10.1006/jmbi.1996.0275
https://doi.org/10.1006/jmbi.1996.0275
Publikováno v:
Biotechnology Letters. 16:1011-1014
Propionic and isobutyric acids were found to be the major inhibitory metabolites for carboxymethyl(CM)-cellulase in high density culture of a recombinantBacillus megaterium pCK108. These organic acids accumulated significantly at the glucose feed rat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2dd748c59a6626c84c3ea7eca87484d5
https://doi.org/10.1007/bf01022394
https://doi.org/10.1007/bf01022394
Publikováno v:
Nucleic Acids Research. 19:4843-4847
Individually inactive N- and C-terminal fragments of the m5C-methyltransferase M.BspRI can complement each other resulting in specific, in vivo methylation of the DNA. This was shown by cloning the coding regions for N- and C-terminal parts of the en
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8afd36f5e30edd1ad990517440f4819
https://doi.org/10.1093/nar/19.18.4843
https://doi.org/10.1093/nar/19.18.4843
Publikováno v:
Journal of Dairy Research. 57:579-585
SummaryThe interactive effect of two of the major Cheddar cheese flavour compounds, H2S and methanethiol, was studied in milk-fat-coated microcapsules containing Brevibacterium linens, cysteine and methionine. Two substrates, cysteine and methionine,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::947cf32a9a68856eb5e1b75cad3de209
https://doi.org/10.1017/s0022029900029629
https://doi.org/10.1017/s0022029900029629
Publikováno v:
Applied microbiology and biotechnology. 58(6)
The tandem multimeric expression of various peptides has been explored by many researchers. However, expression levels have usually not been proportional to the degree of multimerization. To increase the expression level in Escherichia coli of tandem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b641bd6eda1f6759021b7937499847b8
https://pubmed.ncbi.nlm.nih.gov/12021800
https://pubmed.ncbi.nlm.nih.gov/12021800
Publikováno v:
Genetic analysis : biomolecular engineering. 14(3)
The isolation and amplification of large, predetermined segments of a genome from its host have been explored. The prototype of our approach was the excisional replication of some viruses such as the lambda-lysogen. Similar machinery was used to exci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48dda80326e61691c30c4c1dd8011d52
https://pubmed.ncbi.nlm.nih.gov/9526700
https://pubmed.ncbi.nlm.nih.gov/9526700
Publikováno v:
Protein expression and purification. 12(1)
Antimicrobial peptides have received increasing attention as a new pharmaceutical substance, because of their broad spectrum of antimicrobial activities and the rapid development of multidrug-resistant pathogenic microorganisms. The main obstacle to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f9f5b6c24d39bb720a31d6aaef8543e
https://pubmed.ncbi.nlm.nih.gov/9473457
https://pubmed.ncbi.nlm.nih.gov/9473457
Publikováno v:
Biochemical and biophysical research communications. 240(3)
Nitric oxide (NO) from artificial NO donors induces cell death through complete inhibition of mitochondrial respiration of hepatocytes. Treatment of hepatocytes with lipopolysaccharide (LPS) and cytokine mixture (interferon gamma and tumor necrosis f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc22775e1309fd7a80f6b946d2dfbe0d
https://pubmed.ncbi.nlm.nih.gov/9398622
https://pubmed.ncbi.nlm.nih.gov/9398622
Publikováno v:
Genetic analysis : biomolecular engineering. 13(6)
In order to make high-copy-number multimers of DNA fragments in a tandem unit, two different gene amplification vectors (pSK9 and pBBS1) were developed. Two identical class-IIS restriction enzyme sites (BspMI for pSK9 and BbsI for pBBSI) were inverse
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c4b03d408b78a27f3d2b0de43873d56
https://pubmed.ncbi.nlm.nih.gov/9117889
https://pubmed.ncbi.nlm.nih.gov/9117889
Publikováno v:
Gene. 100
A vector was designed to allow predetermined and precise fusion between two cloned genes by constructing a cassette with two unique class-IIS restriction sites, 5′ — ACCTGC-3′ (BspMI) and 5′-CCGGATG-3′ (FokI overlapping with MspI), arranged
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7855b47eba337b99b681c69773d94528
https://pubmed.ncbi.nlm.nih.gov/1647357
https://pubmed.ncbi.nlm.nih.gov/1647357