Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Suminda Hapuarachchi"'
Autor:
Szilan Fodor, Izydor Apostol, Tian Wang, Suminda Hapuarachchi, Xinzhao Grace Jiang, Kenneth Chen, Gang Huang
Publikováno v:
Journal of Pharmaceutical and Biomedical Analysis. 72:59-64
Protein aggregation was observed in a purification intermediate of a therapeutic Fc-fusion protein stored at -30 °C, even though the protein was stable at 4 and -80 °C. The protein was expressed in Escherichia coli as an inclusion body, refolded, a
Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics
Autor:
Suminda Hapuarachchi, Vibha Jawa, Marisa K. Joubert, Qingchun Zhang, Meghana Deshpande, Gregory C. Flynn, Michael Hall
Publikováno v:
The AAPS journal. 18(6)
A potential risk factor for immunogenicity of a biotherapeutic is the low levels of host cell protein (HCP) impurities that remain in the product following the purification process. During process development, significant attention has been devoted t
Autor:
Drew N. Kelner, Xin Zhang, Dave Meriage, Jette Wypych, Jessica Gastwirt, Leszek Poppe, Wojciech Szpankowski, Szilan Fodor, Suminda Hapuarachchi, Xinzhao Grace Jiang, Kenneth Chen, Frank Ye, Gang Huang, Izydor Apostol
Publikováno v:
Pharmaceutical Research
Purpose To predict precision and other performance characteristics of chromatographic purity methods, which represent the most widely used form of analysis in the biopharmaceutical industry. Methods We have conducted a comprehensive survey of purity
Publikováno v:
Analytical Chemistry. 78:3674-3680
We report an online, optical injection interface for capillary zone electrophoresis (CZE) based upon photophysical activation of a caged, fluorogenic label covalently attached to the target analyte. This injection interface allows online analysis of
Publikováno v:
Process Biochemistry. 39:1607-1613
Experiments were carried out to determine the mass balance of selenium biologically processed by a Se-resistant bacterium, Pseudomonas fluorescens K27. The bioprocessing of this toxicant, added to growing liquid cultures, resulted in reduction and in
Publikováno v:
Analytical biochemistry. 414(2)
Overexpression of recombinant Fc fusion proteins in Escherichia coli frequently results in the production of inclusion bodies that are subsequently used to produce fully functional protein by an in vitro refolding process. During the refolding step,
Publikováno v:
Electrophoresis. 28(17)
Here, we report the first utilization of Hadamard transform CE (HTCE), a high-sensitivity, multiplexed CE technique, with photolytic optical gating sample injection of caged fluorescent labels for the detection of biologically important amines. Previ
Publikováno v:
Electrophoresis. 28(7)
We have developed a chemical derivatization scheme for primary amines that couples the fast kinetic properties of o-phthaldialdehyde (OPA) with the photophysical properties of visible, high quantum yield, fluorescent dyes. In this reaction, OPA is us
Publikováno v:
Electrophoresis. 27(20)
We report the utilization of a high power UV light-emitting diode for fluorescence detection (UV-LED-IF) in CE separations. CE-UV-LED-IF allows analysis of a range of environmentally and biologically important compounds, including PAHs and biogenic a
Publikováno v:
Analytical chemistry. 78(5)
We report a new approach for collecting and deconvoluting the data in Hadamard transform capillary electrophoresis, referred to as fast Hadamard transform capillary electrophoresis (fHTCE). Using fHTCE, total analysis times can be reduced by up to 48