Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Sumedha Jayasena"'
Autor:
Larry Gold, Edward N. Brody, Sumedha Jayasena, Jonathan Drew Smith, Dominic Zichi, Michael Willis
Publikováno v:
Molecular Diagnosis. 4:381-388
Backgound : Aptamers are single-stranded oligonucleotides derived from an in vitro evolution protocol called systematic evolution of ligands by exponential enrichment (SELEX). They bind tightly and specifically to target molecules; most aptamers to p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c959f62321dc18c9b5dc3caf4a1f30e
https://doi.org/10.1016/s1084-8592(99)80014-9
https://doi.org/10.1016/s1084-8592(99)80014-9
Autor:
Sumedha Jayasena, Chinh Dang
Publikováno v:
Journal of Molecular Biology. 264:268-278
A random sequence library of single stranded DNA was screened to isolate sequences with high affinity for Thermus aquaticus DNA polymerase (Taq pol), a thermostable enzyme commonly used in the polymerase chain reaction (PCR). Selected oligonucleotide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbf63a3ed13911bd79ab0f428abf47be
https://doi.org/10.1006/jmbi.1996.0640
https://doi.org/10.1006/jmbi.1996.0640
Publikováno v:
Nucleic Acids Research. 24:3407-3414
RNA sequences containing 2'-amino pyrimidines that bind with high-affinity to human thyroid stimulating hormone (hTSH) were isolated from a random sequence library by an in vitro selection-amplification procedure. A representative RNA ligand (T-15) h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e8a1b0611093e69aa9fd4282057c30e
https://doi.org/10.1093/nar/24.17.3407
https://doi.org/10.1093/nar/24.17.3407
Publikováno v:
Nucleic Acids Research. 22:5229-5234
We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::862cb38b2693270f7774d569a6edda88
https://doi.org/10.1093/nar/22.24.5229
https://doi.org/10.1093/nar/22.24.5229
Autor:
Sumedha Jayasena
Publikováno v:
Regulation of Gene Expression by Small RNAs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7ceb859c2942a9dcfaeedd86af01c9cb
https://doi.org/10.1201/9781420008708.ch19
https://doi.org/10.1201/9781420008708.ch19
We have used recombinant human CD4 presented on beads as an affinity matrix to screen a 2'-F-pyrimidine-containing RNA library with a complexity of approximately 10(14) molecules. Affinity-selected aptamers bind recombinant CD4 with low nanomolar equ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a3b387caa118d3d14903d212f35d169
https://europepmc.org/articles/PMC147797/
https://europepmc.org/articles/PMC147797/
Autor:
Sumedha Jayasena, Yun Lin
Publikováno v:
Journal of molecular biology. 271(1)
Single-stranded DNA aptamers that recognize DNA polymerase from Thermus acquaticus (Taq pol) with high affinity have been described recently. These aptamers have been shown to efficiently inhibit the polymerase activity of Taq pol and are useful in e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a5537f7ad547642651d72246772de64
https://pubmed.ncbi.nlm.nih.gov/9300057
https://pubmed.ncbi.nlm.nih.gov/9300057
To investigate the feasibility of using oligonucleotides in flow cytometry we describe a model system consisting of human neutrophil elastase (HNE) coated on 3.3 micro beads and a high affinity DNA ligand for HNE isolated by in vitro selection (SELEX
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be262f92992ee19d6ab6e446a027c383
https://europepmc.org/articles/PMC145686/
https://europepmc.org/articles/PMC145686/
Publikováno v:
Nucleic Acids Research. 28:21e-21
Techniques that provide strong signal amplification are useful in diagnostic applications, especially in detecting low concentrations of non-amplifiable target molecules. A versatile and strong signal amplification method based on activities of a DNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::564dd3b0e7f0cf4840ab7c1433a1cb14
https://doi.org/10.1093/nar/28.7.e21
https://doi.org/10.1093/nar/28.7.e21
Autor:
Michael J. Behe, Sumedha Jayasena
Publikováno v:
Biochemistry. 28:975-980
The double-stranded polypurine.polypyrimidines poly(dG).poly(dC) and poly[d(A-G)].poly[d(T-C)] and the mixed ribose-deoxyribose polynucleotide poly(rG-dC).poly(rG-dC) have been successfully reconstituted into nucleosomes. The radioactively labeled pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e984f2c28fa8ec879b16e27ed9338df4
https://doi.org/10.1021/bi00429a009
https://doi.org/10.1021/bi00429a009