Synaptotagmin 7 docks synaptic vesicles to support facilitation and Doc2α-triggered asynchronous release

Autor: Zhenyong Wu, Grant F Kusick, Manon MM Berns, Sumana Raychaudhuri, Kie Itoh, Alexander M Walter, Edwin R Chapman, Shigeki Watanabe

Publikováno v: eLife, Vol 12 (2024)

Despite decades of intense study, the molecular basis of asynchronous neurotransmitter release remains enigmatic. Synaptotagmin (syt) 7 and Doc2 have both been proposed as Ca2+ sensors that trigger this mode of exocytosis, but conflicting findings ha

Externí odkaz: https://doaj.org/article/a941dafebeac4bb681369387d5e79f94

Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses

Autor: Shuo Li, Sumana Raychaudhuri, Stephen Alexander Lee, Marisa M. Brockmann, Jing Wang, Grant Kusick, Christine Prater, Sarah Syed, Hanieh Falahati, Raul Ramos, Tomas M. Bartol, Eric Hosy, Shigeki Watanabe

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

Action potentials induce synchronous and asynchronous release of neurotransmitters. Here, the authors show that the synchronous and asynchronous release sites are aligned with AMPARs and NMDARs, respectively, in mouse hippocampal synapses. This organ

Externí odkaz: https://doaj.org/article/eaa1dbfabc834db792c456f47bdb3077

Dynamin is primed at endocytic sites for ultrafast endocytosis

Autor: Yuuta Imoto, Sumana Raychaudhuri, Ye Ma, Pascal Fenske, Eduardo Sandoval, Kie Itoh, Eva-Maria Blumrich, Hideaki T. Matsubayashi, Lauren Mamer, Fereshteh Zarebidaki, Berit Söhl-Kielczynski, Thorsten Trimbuch, Shraddha Nayak, Janet H. Iwasa, Jian Liu, Bin Wu, Taekjip Ha, Takanari Inoue, Erik M. Jorgensen, Michael A. Cousin, Christian Rosenmund, Shigeki Watanabe

Publikováno v: Neuron. 110:2815-2835.e13

Dynamin mediates fission of vesicles from the plasma membrane during endocytosis. Typically, dynamin is recruited from the cytosol to endocytic sites, requiring seconds to tens of seconds. However, ultrafast endocytosis in neurons internalizes vesicl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ffe684136bdafe433851d1794d0fde9
https://doi.org/10.1016/j.neuron.2022.06.010

Asynchronous release sites align with NMDA receptors in mouse hippocampal synapses

Autor: Hanieh Falahati, Christine Prater, Raul Ramos, Sumana Raychaudhuri, Shuo Li, Shigeki Watanabe, Tomas M. Bartol, Grant F. Kusick, Jing Wang, Eric Hosy, Marisa M Brockmann, Sarah Syed, Stephen A. Lee

Publikováno v: Nature Communications
Nature Communications, Nature Publishing Group, 2021, 12, pp.677. ⟨10.1038/s41467-021-21004-x⟩
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

Neurotransmitter is released synchronously and asynchronously following an action potential. Our recent study indicates that the release sites of these two phases are segregated within an active zone, with asynchronous release sites enriched near the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba7ed017258b1b1d22397414e4117eae
https://hal.archives-ouvertes.fr/hal-03382653

Correlative Super Resolution and Electron Microscopy to Detect Molecules in Their Native Cellular Context

Autor: Shigeki Watanabe, Grant F. Kusick, Tyler Ogunmowo, Sumana Raychaudhuri, Shuo Li

Publikováno v: Volume Microscopy ISBN: 9781071606902

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6ab92629b6d2cd1dcb065a152b8604c6
https://doi.org/10.1007/978-1-0716-0691-9_1