Application of a fluorescence-based continuous-flow bioassay to screen for diversity of cytochrome P450 BM3 mutant libraries

Autor: Jan N. M. Commandeur, Eduardo Vottero, Jelle Reinen, Suilan Ferman, Nico P. E. Vermeulen

Publikováno v: Reinen, J, Ferman, S, Vottero, E R, Vermeulen, N & Commandeur, J N M 2011, ' Application of a fluorescence-based continuous-flow bioassay to screen for diversity of cytochrome P450 BM3 mutant libraries ', Journal of Biomolecular Screening, vol. 16, no. 2, pp. 239-250 . https://doi.org/10.1177/1087057110394180
Journal of Biomolecular Screening, 16(2), 239-250. SAGE Publications Inc.

A fluorescence-based continuous-flow enzyme affinity detection (EAD) setup was used to screen cytochrome P450 BM3 mutants on-line for diversity. The flow-injection screening assay is based on the BM3-mediated O-dealkylation of alkoxyresorufins formin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::077f835401ae863b5f21aef0dc236776
https://pubmed.ncbi.nlm.nih.gov/21297109