California’s experience with SMA newborn screening: A successful path to early intervention

Autor: Jamie, Matteson, Cindy H, Wu, Deepika, Mathur, Hao, Tang, Stanley, Sciortino, Lisa, Feuchtbaum, Tracey, Bishop, Sudhir C, Sharma, Partha, Neogi, Ina, Fitzgibbon, Richard S, Olney

Publikováno v: Journal of Neuromuscular Diseases. 9:777-785

Background: Universal spinal muscular atrophy (SMA) newborn screening was implemented in California on June 24, 2020. Objective: We describe California’s experience with the first 18 months of SMA newborn screening, including our assay methodology,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fba8fbb1cfbab1c2fe02a806118d56a
https://doi.org/10.3233/jnd-221561

Kinetic Detection of Orthogonal Protein and Chemical Coordinates in Enzyme Catalysis: Double Mutants of Soybean Lipoxygenase

Autor: Sudhir C. Sharma, Judith P. Klinman

Publikováno v: Biochemistry
Biochemistry, vol 54, iss 35

Soybean lipoxygenase-1 (SLO-1) is a paradigmatic enzyme system for studying the contribution of hydrogen tunneling to enzymatic proton-coupled electron transfer processes. In this study, the impact of pairs of double mutants on the properties of SLO-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97e3df0d670910e2de74c37a8501b157
https://doi.org/10.1021/acs.biochem.5b00374

The role of side chain entropy and mutual information for improving the de novo design of Kemp eliminases KE07 and KE70

Autor: Sudhir C. Sharma, Asmit Bhowmick, Hallie Honma, Teresa Head-Gordon

Publikováno v: Physical chemistry chemical physics : PCCP, vol 18, iss 28
Bhowmick, A; Sharma, SC; Honma, H; & Head-Gordon, T. (2016). The role of side chain entropy and mutual information for improving the: De novo design of Kemp eliminases KE07 and KE70. Physical Chemistry Chemical Physics, 18(28), 19386-19396. doi: 10.1039/c6cp03622h. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/5646b2kj

This journal is © 2016 The Owner Societies. Side chain entropy and mutual entropy information between residue pairs have been calculated for two de novo designed Kemp eliminase enzymes, KE07 and KE70, and for their most improved versions at the end

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c2a49815a5d82a90a4cd2b7343ccd84
https://pubmed.ncbi.nlm.nih.gov/27374812

Extremely elevated room-temperature kinetic isotope effects quantify the critical role of barrier width in enzymatic C-H activation

Autor: Sharon Hammes-Schiffer, Alexander D. Scouras, Cody A. Marcus Carr, Judith P. Klinman, Alexander V. Soudackov, Shenshen Hu, Sudhir C. Sharma, Tom Alber

Publikováno v: Journal of the American Chemical Society, vol 136, iss 23
Journal of the American Chemical Society

The enzyme soybean lipoxygenase (SLO) has served as a prototype for hydrogen-tunneling reactions, as a result of its unusual kinetic isotope effects (KIEs) and their temperature dependencies. Using a synergy of kinetic, structural, and theoretical st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d11c0c05b1ef42910d2b88da5d10392
https://escholarship.org/uc/item/3zt303b4

T7 phage display as a method of peptide ligand discovery for PDZ domain proteins

Autor: Adnan Memic, Chamila N. Rupasinghe, Sudhir C. Sharma, Mark R. Spaller, Anne-Cécile E. Duc

Publikováno v: Biopolymers
Biopolymers, Wiley, 2009, 92 (3), pp.183-193. ⟨10.1002/bip.21172⟩

International audience; The use of bacteriophage T7 is presented as a peptide display platform to identify short binding sequences for PDZ domain proteins. Two different domains are examined, the 10th PDZ domain (PDZ10) of the multi-PDZ domain protei

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c1ed5120b3ae1e17bdec5d47a7a6299
https://hal.archives-ouvertes.fr/hal-01668582