Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Sudheerbabu Soorapaneni"'
Autor:
Veena A. Raiker, Sriram Padmanabhan, Sudheerbabu Soorapaneni, Jitendra Kumar, Prakash Kotwal, Ketaki Sabnis-Prasad, Anjali Apte-Deshp
Publikováno v:
Journal of Microbial & Biochemical Technology. :038-045
An arabinose promoter based expression system in E. coli for the production and purification of recombinant human growth hormone (rhGH) was designed and implemented. The shake flask studies indicated appreciable amounts of rhGH expressed in modified
Autor:
Veena A. Raiker, Rahul Nair, Prakash Kotwal, Sriram Padmanabhan, Sudheerbabu Soorapaneni, Pankaj Salvi, Sampali Banerjee, Suman Bandyopadhyay
Publikováno v:
New Biotechnology. 26:282-288
We report a simple and cost-effective autoinducible media component responsible for the autoinduction of proteins in Escherichia coli under lacUV5 promoter system. Yeast extract (YE) at high concentration was found to stimulate the expression of T7 R
Autor:
Goutam Mandal, Sriram Padmanabhan, Anjali Deepak Apte-Deshpande, Sandeep Somani, Sudheerbabu Soorapaneni
Publikováno v:
Journal of Biotechnology. 143:44-50
Recombinant human granulocyte colony stimulating factor (rhGCSF) was expressed in methylotrophic yeast Pichia pastoris under the control of AOX1 promoter after integration of the GCSF gene into P. pastoris genome. Methanol induction of the Pichia int
Autor:
Goutam Mandal, Sriram Padmanabhan, Prakash Kotwal, Bhaskarjyoti Prasad, Sachin Rewanwar, Nagnath Mandi, Sudheerbabu Soorapaneni
Publikováno v:
Protein Expression and Purification. 64:69-75
Staphylokinase (SAK) is emerging as an important thrombolytic agent. In this report, we describe the cloning, expression, purification and activity studies of the SAK gene of Staphylococcus aureus from a custom synthesised SAK gene. The SAK gene of 4
Autor:
Veena A. Raiker, Ketaki Sabnis Prasad, Sudheerbabu Soorapaneni, Shardul Salunkhe, Sriram Padmanabhan
Publikováno v:
Protein expression and purification. 71(2)
The human interferon alpha 2b (IFN alpha2b) belongs to the interferon family of cytokines that exerts many biological functions like inhibition of virus multiplication, repression of tumour growth and other immunological functions. Herein, a syntheti
Autor:
Bhaskarjyoti Prasad, Jitendra Kumar, Sudheerbabu Soorapaneni, Goutam Mandal, Sriram Padmanabhan, Anjali Apte-Deshpnade
Publikováno v:
Biotechnology letters. 31(6)
Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SA
Autor:
Prakash Kotwal, Rahul Nair, P. Salvi, Sriram Padmanabhan, Sampali Banerjee, Veena A. Raiker, Sudheerbabu Soorapaneni
Publikováno v:
New Biotechnology. 25:S203