Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Sudarson Sundarrajan"'
Autor:
Anupama Cheleri Neduvat, Prerana Madhusudhana Murthy, Sudarson Sundarrajan, Sriram Padmanabhan
Publikováno v:
MethodsX, Vol 5, Iss , Pp 881-889 (2018)
Genomic DNA (gDNA) obtained from whole blood samples is a critical element for genomic research and clinical diagnosis. PCR efficiencies of the targeted genes like HLA-A, -B, -C, DPB1 and DRB1 using such isolated gDNAs were variable in spite of havin
Externí odkaz:
https://doaj.org/article/d3f237dfa4c141e294e44c509c22271b
Publikováno v:
Biotechnology Reports, Vol 19, Iss , Pp - (2018)
Using Sso7d from Sulfolobus solfataricus as the DNA binding protein fused to Taq DNA polymerase at its amino terminus, we report the hyper-expression and a novel purification methodology of Sso7d-Taq polymerase (S-Taq) using aqueous two-phase extract
Externí odkaz:
https://doaj.org/article/2ed04407527c4e3b9f1f244749e28360
Autor:
Prerana Madhusudhana Murthy, Sudarson Sundarrajan, Anupama Cheleri Neduvat, Sriram Padmanabhan
Publikováno v:
Walailak Journal of Science and Technology (WJST). 17:139-153
Polymerase chain reaction (PCR)-based technology for clinical HLA typing involves DNA Sanger sequencing of the PCR amplified products of polymorphic loci of HLA, such as HLA-A, -B, -C, -DPB1,-DQB1, and -DRB1, in support of unrelated donor hematopoiet
Autor:
Sriram Padmanabhan, Sudarson Sundarrajan, Cheemalamarri Veenadhar, Prerana Madhusudhana Murthy, Anupama Cheleri Neduvat
Publikováno v:
Walailak Journal of Science and Technology (WJST). 17:113-127
Genomic DNA extracted from human saliva samples showed high inter-subject variations in DNA yield, compelling the need to explore a methodology for the accurate quantitation of the extracted genomic DNA. Quantitative assessment of DNA extracted from
Publikováno v:
Journal of Maxillofacial and Oral Surgery. 17:254-259
INTRODUCTION: Oral submucosal fibrosis (OSMF) is a chronic debilitating fibrotic disease of the oral cavity and is a serious health hazard in south Asia and, increasingly, the rest of the world. The molecular basis behind various treatment modalities
Publikováno v:
Journal of Clinical and Translational Research
Background: Urethral stricture disease (USD) is effectively managed by buccal mucosa (BM) urethroplasty. Lack of adequate healthy BM has led to the use of autologous tissue-engineered BM grafts. Such grafts are costly, not easily scalable and recurre
Publikováno v:
Brazilian Journal of Microbiology, Volume: 49, Issue: 4, Pages: 848-855, Published: DEC 2018
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology, Vol 49, Iss 4, Pp 848-855
Brazilian Journal of Microbiology v.49 n.4 2018
Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology, Vol 49, Iss 4, Pp 848-855
Brazilian Journal of Microbiology v.49 n.4 2018
Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escher
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9db7b172b7285b31a69efab08b435d47
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400848&lng=en&tlng=en
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400848&lng=en&tlng=en
Autor:
Sriram Padmanabhan, Prerana Madhusudhana Murthy, Anupama Cheleri Neduvat, Sudarson Sundarrajan
Publikováno v:
MethodsX, Vol 5, Iss, Pp 881-889 (2018)
MethodsX
MethodsX
Graphical abstract
Genomic DNA (gDNA) obtained from whole blood samples is a critical element for genomic research and clinical diagnosis. PCR efficiencies of the targeted genes like HLA-A, -B, -C, DPB1 and DRB1 using such isolated gDNAs were va
Genomic DNA (gDNA) obtained from whole blood samples is a critical element for genomic research and clinical diagnosis. PCR efficiencies of the targeted genes like HLA-A, -B, -C, DPB1 and DRB1 using such isolated gDNAs were va
Autor:
Anupama Byrappa Ramalinga, Appaiah Chemira Biddappa, Bharathi Sriram, Sudarson Sundarrajan, Sriram Padmanabhan
Publikováno v:
Biotechnology and Applied Biochemistry. 59:495-502
The development of lytic bacteriophages as therapeutic products is an attractive alternative to antibiotics. In this study, we evaluated the potential of phage tails for lysing Gram-positive bacteria. Phage P954, a well-characterized temperate staphy
Autor:
Sriram Padmanabhan, S. Nandini, Sudarson Sundarrajan, Sukumar Hariharan, Vivek Daniel Paul, Bharathi Sriram, R. Sanjeev Saravanan, G.P. Nirmal Kumar
Publikováno v:
Virus Research. 169:182-187
We demonstrate that the prophage status of bacteria plays a critical role in achieving homogenous population of a phage preparation. When a lytic Staphylococcus bacteriophage 44AHJD was propagated in a Staphylococcus clinical isolate, the enriched ph