Zobrazeno 1 - 10
of 1 929
pro vyhledávání: '"Subcloning"'
Autor:
Marcus Weinguny, Gerald Klanert, Peter Eisenhut, Andreas Jonsson, Daniel Ivansson, Ann Lövgren, Nicole Borth
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 18, Iss , Pp 1320-1329 (2020)
Chinese Hamster Ovary (CHO) cells are the working horse of the pharmaceutical industry. To obtain high producing cell clones and to satisfy regulatory requirements single cell cloning is a necessary step in cell line development. However, it is also
Externí odkaz:
https://doaj.org/article/232d6707f27a4be9977614512c8eb79d
Akademický článek
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Publikováno v:
Viruses, Vol 14, Iss 8, p 1800 (2022)
HepG2 cells reconstituted with Hepatitis B virus (HBV) entry receptor sodium taurocholate co-transporting polypeptide (NTCP) are widely used as a convenient in vitro cell culture infection model for HBV replication studies. As such, it is pertinent t
Externí odkaz:
https://doaj.org/article/7620855d355c40d8923d5dd22ca7895e
Autor:
Soheila Rahnamaei Yahyaabadi., Hossein Honari, Masoud Abdollahi, Seyed Mojtaba Aghaie, Mohamadali Ebrahimi
Publikováno v:
Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 12, Iss 12, Pp 42-52 (2019)
Background and Objectives: Extract of mistletoe lectin (Viscum album L) leaves contains MLI protein that is a type 2 ribosome-inactivating protein (RIP2) with lectin properties. Shiga toxin (STxB) has been considered as an immunoadjuvant and carrier,
Externí odkaz:
https://doaj.org/article/acdc314935d741ffbf7bcbe34c7350b5
Publikováno v:
Brazilian Archives of Biology and Technology, Vol 63 (2020)
Abstract Gene subcloning, a process in which the nucleotide sequence of interest is excised from on plasmid and inserted into another, seems to be an easy task to done. However, not all subcloning attempts are successful, even when the insert sequenc
Externí odkaz:
https://doaj.org/article/223f1e3ec802458b9407ec60274b91ab
Autor:
Roland S. Hilgarth, Thomas M. Lanigan
Publikováno v:
MethodsX, Vol 7, Iss , Pp 100759- (2020)
PCR is a powerful tool for generating specific fragments of DNA that can be used to create gene variations or tagged expression constructs. Overlap extension PCR is a valuable technique that is commonly used for cloning large complex fragments, makin
Externí odkaz:
https://doaj.org/article/02affab4f25e48c59f4f608459ecca81
Publikováno v:
Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 11, Iss 10, Pp 30-39 (2017)
Background and Objectives: Burkholderia pseudomallei is the cause of the melioidosis disease in human, and is transmitted to patients through oral route, inhalation, or skin scratch. This agent is classified as a category B in the classification of b
Externí odkaz:
https://doaj.org/article/4e4d14a652f841398911f882b9015350
Publikováno v:
Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 10, Iss 6, Pp 13-23 (2016)
Background and Objectives: Botulism syndrome is caused by Clostridium botulinum neurotoxin. This neurotoxin has seven serotypes ranging from A to G. The best way to prevent botulism syndrome caused by Botulinum neurotoxin (BoNT), is using recombinant
Externí odkaz:
https://doaj.org/article/8b4615e04c464581a4180e32e44a29b8
Autor:
Katsuko Komatsu, Naoko Anjiki, Nobuo Kawahara, Akihito Takano, Shigeki Hayashi, Qundong Liu, Shu Zhu
Publikováno v:
Journal of Natural Medicines. 76:276-280
Intron length polymorphism (ILP) markers in genes encoding diketide-CoA synthase (DCS) and curcumin synthase (CURS) showed high identification rates in 13 Curcuma species from Asia. However, the sequences of the intron regions have not yet been analy
Autor:
Peter Eisenhut, Andreas Jonsson, Nicole Borth, Daniel Ivansson, Gerald Klanert, Ann Lövgren, Marcus Weinguny
Publikováno v:
Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Vol 18, Iss, Pp 1320-1329 (2020)
Computational and Structural Biotechnology Journal, Vol 18, Iss, Pp 1320-1329 (2020)
Graphical abstract By repeatedly selecting and pooling the most rapidly outgrowing clones during multiple single cell subcloning rounds, the time required for subcloning was reduced from 3 to 2 weeks and the cloning efficiency significantly increased