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NeIle, a Genetically Encoded Indicator for Branched-Chain Amino Acids Based on mNeonGreen Fluorescent Protein and LIVBP Protein.

Autor: Asanova AN; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Subach OM; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Myachina SA; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Evteeva MA; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Gunitseva NM; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Borisova AA; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Patrushev MV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Vlaskina AV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Nikolaeva AY; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Yang L; School of Life Sciences, Westlake University, Hangzhou 310024, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, China.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou 310024, China., Gabdulkhakov A; Institute of Protein Research, Russian Academy of Sciences, Pushchino 142290, Russia., Dronova E; Moscow Insitite of Physics and Technology, Dolgoprudny 141700, Russia., Samygina VR; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia., Xiao X; School of Life Sciences, Westlake University, Hangzhou 310024, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, China.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou 310024, China., Zhao H; Chinese Institute for Brain Research, Beijing 102206, China., Piatkevich KD; School of Life Sciences, Westlake University, Hangzhou 310024, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou 310024, China.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou 310024, China., Subach FV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow 123182, Russia.

Publikováno v: ACS sensors [ACS Sens] 2024 Oct 25; Vol. 9 (10), pp. 5135-5147. Date of Electronic Publication: 2024 Oct 14.

NeMeHg, genetically encoded indicator for mercury ions based on mNeonGreen green fluorescent protein and merP protein from Shigella flexneri .

Autor: Subach OM; Complex of NBICS Technologies, National Research Center, Kurchatov Institute, Moscow, Russia., Piatkevich KD; School of Life Sciences, Westlake University, Hangzhou, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China., Subach FV; Complex of NBICS Technologies, National Research Center, Kurchatov Institute, Moscow, Russia.

Publikováno v: Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2024 Jul 10; Vol. 12, pp. 1407874. Date of Electronic Publication: 2024 Jul 10 (Print Publication: 2024).

Bright and stable monomeric green fluorescent protein derived from StayGold.

Autor: Zhang H; School of Life Sciences, Westlake University, Hangzhou, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China., Lesnov GD; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, Russia., Subach OM; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, Russia., Zhang W; School of Life Sciences, Westlake University, Hangzhou, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China., Kuzmicheva TP; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, Russia., Vlaskina AV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, Russia., Samygina VR; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, Russia.; Institute of Crystallography of Federal Research Scientific Center 'Crystallography and Photonics' of the Russian Academy of Sciences, Moscow, Russia., Chen L; Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing, China.; PKU-IDG/McGovern Institute for Brain Research, Beijing, China., Ye X; Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing, China., Nikolaeva AY; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, Russia., Gabdulkhakov A; Institute of Protein Research, Russian Academy of Sciences, Pushchino, Russia., Papadaki S; School of Life Sciences, Westlake University, Hangzhou, China.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China., Qin W; National Facility for Protein Science in Shanghai, Shanghai Advanced Research Institute CAS, Shanghai, China., Borshchevskiy V; Moscow Institute of Physics and Technology, Dolgoprudnyi, Russia., Perfilov MM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Gavrikov AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Drobizhev M; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Mishin AS; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Piatkevich KD; School of Life Sciences, Westlake University, Hangzhou, China. kiryl.piatkevich@westlake.edu.cn.; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China. kiryl.piatkevich@westlake.edu.cn.; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, China. kiryl.piatkevich@westlake.edu.cn., Subach FV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, Russia. subach_fv@nrcki.ru.

Publikováno v: Nature methods [Nat Methods] 2024 Apr; Vol. 21 (4), pp. 657-665. Date of Electronic Publication: 2024 Feb 26.

FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.

Autor: Subach OM; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, 123182, Russia. Electronic address: subach_om@nrcki.ru., Varfolomeeva L; Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia. Electronic address: l.varfolomeeva@fbras.ru., Vlaskina AV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, 123182, Russia. Electronic address: vlaskina_av@nrcki.ru., Agapova YK; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, 123182, Russia. Electronic address: agapova.jk@gmail.com., Nikolaeva AY; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, 123182, Russia; Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia. Electronic address: nikolaeva_ay@nrcki.ru., Piatkevich KD; School of Life Sciences, Westlake University, Hangzhou, 310024, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, 310024, China; Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, 310024, China. Electronic address: kiryl.piatkevich@westlake.edu.cn., Patrushev MV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, 123182, Russia. Electronic address: patrushev_mv@nrcki.ru., Boyko KM; Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia. Electronic address: kmb@inbi.ras.ru., Subach FV; Complex of NBICS Technologies, National Research Center 'Kurchatov Institute', Moscow, 123182, Russia. Electronic address: subach_fv@nrcki.ru.

Publikováno v: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Jul 12; Vol. 665, pp. 169-177. Date of Electronic Publication: 2023 Apr 28.